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Cytotoxic effects of sera from patients with systemic scleroderma: Comparison of three different in vitro methods

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Summary

Sera from 93 patients with systemic scleroderma including incipient or prodromal acroscleroderma and from 43 healthy individuals were studied for cytotoxic effects on endothelial cells by means of three different in vitro methods. Inhibition of3H-thymidine incorporation by endothelium was caused by about 33% of sera, almost exclusively from patients with diffuse scleroderma and the transitional form: acroscleroderma—diffuse scleroderma. An antibody-dependent cellular cytotoxicity assay revealed cytotoxicity of about 37% of sera from patients with diffuse sceroderma and the transitional form but also of a proportion of sera from patients with CREST syndrome with pronounced vascular changes. Serum cytotoxic activity, revealed by both methods, was related with more frequent involvement of muscle and kidney in the patients. A direct51Cr release assay showed the cytotoxicity only in 4 of 68 cases in diffuse scleroderma with pronounced internal organ involvement. Thus, depending on the method used, various types of cytotoxicity could be detected in sera from patients with systemic scleroderma.

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Authors and Affiliations

  1. Department of Dermatology, Warsaw School of Medicine, Koszykowa 82A, 02-008, Warsaw, Poland

    S. Majewski, M. Blaszczyk, S. Jabłonska, L. Rudnicka, A. Skiendzielewska & B. Makiela

  2. Transplantation Institute, Warsaw School of Medicine, Warsaw, Poland

    M. Wasik

Authors
  1. S. Majewski
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  2. M. Blaszczyk
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  3. S. Jabłonska
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  4. L. Rudnicka
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  5. M. Wasik
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  6. A. Skiendzielewska
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  7. B. Makiela
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Majewski, S., Blaszczyk, M., Jabłonska, S. et al. Cytotoxic effects of sera from patients with systemic scleroderma: Comparison of three different in vitro methods. Rheumatol Int 10, 65–70 (1990). https://doi.org/10.1007/BF02274785

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  • DOI: https://doi.org/10.1007/BF02274785

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