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Relationship between proteolipids and calcium-phospholipid-phosphate complexes inBacterionema matruchotii calcification

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Summary

Calcium-phospholipid-phosphate complexes (Ca-PL-P) were isolated from calcified and uncalcifiedBacterionema matruchotii and its calcified lipid extracts. Similar complexes were absent from the noncalcifying bacteriumActinomyces naeslundii. The majority of the Ca-PL-P complexes were associated with the proteolipid acidic phospholipid component. Ca-PL-P complexes isolated fromB. matruchotii and from calcified proteolipid contained phosphatidylinositol, phosphatidylinositol-4-phosphate, phosphatidylinositol-4,5-diphosphate, and phosphatidylserine. They consisted of approximately 52 mole % Ca, 32 mole % organic P, and 15 mole % Pi. During Ca-PL-P extraction fromB. matruchotii or its proteolipid-containing calcified lipid extracts, the proteolipid was dissociated and the apoprotein precipitated as fluff at the aqueous-organic solvent interface, thus explaining the failure to detect protein in Ca-PL-P preparations. When the ability of Ca-PL-P complexes and lipid fractions ofB. matruchotii to initiate apatite formation from metastable calcium phosphate solution was compared, the yield of hydroxyapatite decreased as follows: Ca-PL-P > proteolipid acidic phospholipids > proteolipid > crude phospholipid > total lipids > whole cells.

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References

  1. Irving, J.: Theories of mineralization of bone, Clin. Orthop. Rel. Res.97:225–236, 1973

    Google Scholar 

  2. Irving, J., Wuthier, R.E.: Histochemistry and biochemistry of calcification with special reference to the role of lipids, Clin. Orthop. Rel. Res.56:237–250, 1968

    CAS  Google Scholar 

  3. Wuthier, R.E.: Lipids of mineralizing epiphyseal tissues in the bovine fetus, J. Lipid Res.9:68–78, 1968

    CAS  PubMed  Google Scholar 

  4. Odutuga, A., Prout, R.E.S.: Lipid analysis of human enamel and dentine, Arch. Oral Biol.19:729–731, 1974

    Article  CAS  PubMed  Google Scholar 

  5. Wuthier, R.E.: Zonal analysis of phospholipids in the epiphyseal cartilage and bone of normal and rachitic chickens and pigs, Calcif. Tissue Res.8:36–53, 1971

    Article  CAS  PubMed  Google Scholar 

  6. Shapiro, I.M.: The association of phospholipids with anorganic bone, Calcif. Tissue Res.5:13–20, 1970

    Article  CAS  PubMed  Google Scholar 

  7. Ennever, J., Vogel, J.J., Levy, B.M.: Lipid and bone matrix calcification in vitro, Proc. Soc. Exp. Biol. Med.145:1386–1388, 1974

    CAS  PubMed  Google Scholar 

  8. Boskey, A.L., Posner, A.S.: The role of synthetic and bone extracted Ca-phospholipid-PO4 complexes in hydroxyapatite formation, Calcif. Tissue Res.23:251–258, 1977

    Article  CAS  PubMed  Google Scholar 

  9. Anderson, H.C.: Matrix vesicle calcification, Fed. Proc.35:105–108, 1976

    CAS  Google Scholar 

  10. Boskey, A.L.: Models of matrix vesicle calcification, Inorg. Perspect. Biol. Med.2:51–92, 1979

    Google Scholar 

  11. Wuthier, R.E.: Lipid composition of isolated epiphyseal cartilage cells, membranes and matrix vesicles, Biochem. Biophys. Acta409:128–143, 1975

    CAS  PubMed  Google Scholar 

  12. Peress, N.S., Anderson, H.C., Sajdera, S.W.: The lipid of matrix vesicles from bovine fetal epiphyseal cartilage, Calcif. Tissue Res.14:275–281, 1974

    Article  CAS  PubMed  Google Scholar 

  13. Ennever, J., Vogel, J.J., Rider, L.J., Boyan-Salyers, B.: Nucleation of microbiologic calcification by proteolipid, Proc. Soc. Exp. Biol. Med.152:147–150, 1976

    CAS  PubMed  Google Scholar 

  14. Ennever, J., Vogel, J., Rider, L., Paoloski, S.: Proteolipid and calculus matrix in vitro, J. Dent. Res.56:140–142, 1977

    CAS  PubMed  Google Scholar 

  15. Ennever, J., Boyan-Salyers, B., Riggan, L.: Proteolipid and bone matrix calcification in vitro, J. Dent. Res.57:731–734, 1978

    CAS  PubMed  Google Scholar 

  16. Boskey, A.L., Posner, A.S.: Extraction of a calcium-phospholipid-phosphate complex from bone, Calcif. Tissue Res.19:273–283, 1976

    CAS  PubMed  Google Scholar 

  17. Boskey, A.L., Goldberg, M.R., Posner, A.S.: Calciumphospholipid-phosphate complexes in mineralizing tissue, Proc. Soc. Exp. Biol. Med.157:588–591, 1977

    Google Scholar 

  18. Ennever, J., Vogel, J., Brown, L.: Survey of micro-organisms for calcification in a synthetic medium, J. Dent. Res.51:1483–1486, 1972

    CAS  PubMed  Google Scholar 

  19. Ennever, J., Riggan, L., Vogel, J., Boyan-Salyers, B.: Characterization ofBacterionema matruchotii calcification nucleator, J. Dent. Res.57:637–642, 1978

    CAS  PubMed  Google Scholar 

  20. Getz, G.S., Jakovcic, S., Heywood, J., Frank, J., Rabinowitz, M.: A two dimensional thin-layer chromatographic system for phospholipid separation. The analysis of yeast phospholipids, Biochem. Biophys. Acta218:441–453, 1970

    CAS  PubMed  Google Scholar 

  21. Cotmore, J.M., Nichols, G., Wuthier, R.E.: Phospholipidcalcium-phosphate complex. Enhanced calcium migration in the presence of phosphate, Science172:1339–1341, 1971

    CAS  PubMed  Google Scholar 

  22. Palmer, F., Verpoorte, J.: The phospholipid component of solubilized erythrocyte membrane protein, Can. J. Biochem.49:337–347, 1971

    Article  CAS  PubMed  Google Scholar 

  23. Fiske, C.V., Subbarow, Y.: The colorimetric determination of phosphorus, J. Biol. Chem.66:375–400, 1925

    CAS  Google Scholar 

  24. Axelrod, J., Reichenthal, J., Brodie, B.B.: The direct determination of phosphatidyl ethanolamine and phosphatidylserine in plasma and red blood cells, J. Biol. Chem.204:903–911, 1953

    CAS  PubMed  Google Scholar 

  25. Horning, M., Murikami, S., Horning, E.: Analysis of phospholipids, ceramides and cerebrosides by gas chromatography-mass spectometry, Am. J. Clin. Nutr.24:1086–1096, 1971

    CAS  PubMed  Google Scholar 

  26. Willis, J.B.: The determination of metals in blood serum by atomic absorption spectroscopy. I. Calcium, Sepctrochim. Acta16:259–272, 1960

    Article  CAS  Google Scholar 

  27. Crouch, S.R., Malmstadt, H.V.: A mechanistic investigation of molybdenum blue method for determination of phosphate, Anal. Chem.39:1084–1089, 1967

    Article  CAS  Google Scholar 

  28. Gunsalis, I.C., Stamer, J.R.: Transaminases in bacteria, Methods Enzymol.2:170–177, 1955

    Article  Google Scholar 

  29. Lowry, O., Roseborough, N., Farr, A., Randell, R.: Protein measurement with the Folin phenol reagent, J. Biol. Chem.193:265–275, 1951

    CAS  PubMed  Google Scholar 

  30. Boskey, A.L., Posner, A.S.: Formation of hydroxyapatite at low supersaturation, J. Phys. Chem.80:40–45, 1976

    Article  CAS  Google Scholar 

  31. Nancollas, G., Tomazic, B.: Growth of calcium phosphate on hydroxyapatite crystals. Effect of supersaturation and ionic medium, J. Phys. Chem.78:2218–2224, 1974

    Article  CAS  Google Scholar 

  32. Boyan-Salyers, B., Vogel, J.J., Ennever, J.: Pre-apatite mineral deposition inBacterionema matruchotii, J. Dent. Res.57:291–295, 1978

    CAS  PubMed  Google Scholar 

  33. Wuthier, R.E.: Electrolytes of isolated epiphyseal chrondocytes, matrix vesicles and extracellular fluid, Calcif. Tissue Res.23:125–133, 1977

    Article  CAS  PubMed  Google Scholar 

  34. Ennever, J., Vogel, J.J., Riggan, L., Boyan-Salyers, B.: Characterization of calculus matrix calcification nucleator, J. Dent. Res.58:619–623, 1979

    CAS  PubMed  Google Scholar 

  35. Ennever, J., Vogel, J., Riggan, L.: Phospholipids of a bone matrix calcification nucleator, J. Dent. Res.57:731–734, 1978

    CAS  PubMed  Google Scholar 

  36. Folch-Pi, J., Stoffyn, P.: Proteolipids from membrane systems, N. Y. Acad. Sci.195:86–107, 1972

    CAS  Google Scholar 

  37. Folch-Pi, J., Sakura, J.: Preparation of the proteolipid apoprotein from bovine heart, liver and kidney, Biochim. Biophys. Acta427:410–427, 1976

    CAS  PubMed  Google Scholar 

  38. Boggs, J.M., Wood, D.D., Moscarello, M.A., Papahadjopoulos, D.: Lipid phase separation induced by a hydrophobic protein in phosphatidyl choline vesicles, Biochemistry16:2325–2329, 1977

    Article  CAS  PubMed  Google Scholar 

  39. Vogel, J., Boyan-Salyers, B., Campbell, M.: Protein-phospholipid interactions in biologic calcification, Metab. Bone Dis. Rel. Res.1:149–153, 1978

    Article  CAS  Google Scholar 

  40. Boyan-Salyers, B., Vogel, J., Riggan, L., Summers, F., Howell, R.: Application of a microbial model to biologic calcification, Metab. Bone Dis. Rel. Res.1:143–148, 1978

    Article  CAS  Google Scholar 

  41. Wuthier, R., Gore, S.: Partition of inorganic ions and phospholipids in isolated cell, membrane and matrix vesicle fractions. Evidence for Ca:Pi:acidic phospholipid complexes, Calcif. Tissue Res.24:163–171, 1977

    Article  CAS  PubMed  Google Scholar 

  42. Ali, S.: Analysis of matrix vesicles and their role in the calcification of epiphyseal cartilage, Fed. Proc.35:135–142, 1976

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. University of Texas Dental Science Institute, 77025, Houston, Texas

    B. D. Boyan-Salyers & A. L. Boskey

  2. Hospital for Special Surgery, Cornell University Medical School, New York, New York, USA

    B. D. Boyan-Salyers & A. L. Boskey

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  1. B. D. Boyan-Salyers
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  2. A. L. Boskey
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Boyan-Salyers, B.D., Boskey, A.L. Relationship between proteolipids and calcium-phospholipid-phosphate complexes inBacterionema matruchotii calcification. Calcif Tissue Int 30, 167–174 (1980). https://doi.org/10.1007/BF02408622

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  • DOI: https://doi.org/10.1007/BF02408622

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