Summary
The skin comprises three layers: epidermis, dermis, and hypodermis. We report here on a skin, reconstructed in vitro, that is composed of all three layers. The topmost layer, epidermis, was exposed to air by a new method. The exposure induced an extensive proliferation, and differentiation, i.e. keratinization was eventually observed in the cultured epidermal cells. Skin thus cultured will be a useful graft of transplantation and provide an ideal model system in which to study diseases of the skin.
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Sugihara, H., Toda, S., Miyabara, S. et al. Reconstruction of the skin in three-dimensional collagen gel matrix culture. In Vitro Cell Dev Biol - Animal 27, 142–146 (1991). https://doi.org/10.1007/BF02631000
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DOI: https://doi.org/10.1007/BF02631000