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The effect of recombinant human basic fibroblast growth factor rhfgf-2 on human osteoblast in growth and phenotype expression

  • Growth, Differentiation And Senescence
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Abstract

This paper describes the studies of human recombinant basic fibroblast growth factor (rhFGF-2) for its effects on human osteoblast growth and phenotype expression. During a 24-h period of treatment, rhFGF-2 highly stimulated DNA synthesis in a dose-related fashion with a maximum stimulation of 150% for 1 ng/ml. On the other hand, rhFGF-2 decreases alkaline phosphatase activity, synthesis of type I collagen, and cumulative amount of osteocalcin. Moreover, rhFGF-2 provoked a threefold increase in the amount of intracellular cAMP. Scatchard plots show the presence of two classes of [125I] rhFGF-2 receptors. This data suggests that rhFGF-2 which stimulate cell replication may act indirectly as an anabolic agent and stimulate some of the phenotypic expression markers.

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Authors and Affiliations

  1. INSERM-U. 306, Université de Bordeaux II, 146 rue Léo Saignat, 33076, Bordeaux Cédex, France

    Saadia Berrada, Francoise Lefebvre & Marie-Francoise Harmand

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  1. Saadia Berrada
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  2. Francoise Lefebvre
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  3. Marie-Francoise Harmand
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Berrada, S., Lefebvre, F. & Harmand, MF. The effect of recombinant human basic fibroblast growth factor rhfgf-2 on human osteoblast in growth and phenotype expression. In Vitro Cell Dev Biol - Animal 31, 698–702 (1995). https://doi.org/10.1007/BF02634091

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  • DOI: https://doi.org/10.1007/BF02634091

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