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The effect of mechanical strain on fetal rat lung cell proliferation: Comparison of two-and three-dimensional culture systems

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Summary

Normal growth of the fetal lung is dependent on fetal breathing movements. We have previously reported that an intermittent strain, which simulates normal fetal breathing movements, stimulates DNA synthesis and cell division of mixed fetal rat lung cells maintained in organotypic cultures. To examine which cell type is responding to mechanical strain and to investigate whether the effects of strain on cell proliferation and mechanotransduction are affected by tissue architecture, we isolated fetal lung cells and subjected them to intermittent strain either as two-dimensional monolayer cultures or as three-dimensional organotypic cultures. Strain enhanced DNA synthesis of mixed cells, epithelial cells, and fibroblasts when cultured in a three-dimensional configuration. In contrast, no stimulatory effect on cell proliferation was observed depending on the culture conditions. These results suggest that mechanical strain stimulates the proliferation of both epithelial cells and fibroblasts and that the response of fetal lung cells to mechanical strainin vitro depends on cellular architecture.

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Authors and Affiliations

  1. The Medical Research Council Group in Lung Development, Neonatal Research Division, Hospital for Sick Children Research Institute, Department of Paediatrics, University of Toronto, M5G 1X8, Toronto, Ontario, Canada

    Mingyao Liu, Jing Xu, Patricia Souza, Bryony Tanswell, A. Keith Tanswell & Martin Post

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  1. Mingyao Liu
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  2. Jing Xu
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  3. Patricia Souza
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  6. Martin Post
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Liu, M., Xu, J., Souza, P. et al. The effect of mechanical strain on fetal rat lung cell proliferation: Comparison of two-and three-dimensional culture systems. In Vitro Cell Dev Biol - Animal 31, 858–866 (1995). https://doi.org/10.1007/BF02634570

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  • DOI: https://doi.org/10.1007/BF02634570

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