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Protective effects of GSH, vitamin E, and selenium on lipid peroxidation in cadmium-fed rats

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Abstract

Increased intake of Cd results in its retention and in peroxidative damage in soft tissues. Coadministration of antioxidants, viz., glutathione (GSH), α-tocopherol, and Se, restricted the uptake and distribution of Cd in liver and kidney of rats. Moreover, no rise in malondialdehyde was recorded. Although possible antioxidative mechanisms manifested by GSH, α-tocopherol, and Se have been discussed, it is hypothesized that GSH functions as a Cd chelator. Glutathione yielded favorable effects in comparison to Se and α-tocopherol.

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References

  1. L. Friberg, C. G. Elinder, T. Kjellstrom, and G. F. Nordberg,Cadmium and Health. A Toxicological and Epidemiological Appraisal, CRC, Boca Raton, FL (1986).

    Google Scholar 

  2. M. Webb, Binding of cadmium ions by rat liver and kidney,Biochem. Pharmacol. 21, 2751 (1972).

    Article  PubMed  CAS  Google Scholar 

  3. F. A. Probst, W. F. Bousquet, and T. S. Miya, Correlation of hepatic metallo thionein concentrations with acute cadmium toxicity in the mouse,Toxicol. Appl. Pharmacol. 39, 61 (1977).

    Article  PubMed  CAS  Google Scholar 

  4. S. V. S. Rana, V. P. Agrawal, and N. G. Bharadwaj, Cadmium and Zinc induced changes in the liver and kidney of rats,Int. J. Tiss. Reac. 2, 57–64 (1980).

    Google Scholar 

  5. R. W. Chen, P. A. Wanger, W. G. Hockstra, and H. E. Ganather, Affinity labelling studies with cadmium induced testicular injury in the rats.J. Reprod. Fertility 38, 293 (1974).

    Article  CAS  Google Scholar 

  6. P. D. Whanger, Selenium versus metal toxicity in animals, in “Proc. Symposium Selenium, Tellurium in the environment,” Industrial Health Foundation: Pittsburgh, P A, pp. 234 (1976).

    Google Scholar 

  7. H. D. Stove, Biliary excretion of cadmium by rats, effects of zinc, cadmium and selenium pretreatments,J. Toxicol Environ Health 2, 45–53 (1976).

    Google Scholar 

  8. B. A. Arrick, C. F. Nathan, and Z. A. Cohn, Glutathione metabolism as a determinant of therapeutic efficacy. A review:Cancer Res. 44, 4334 (1984).

    Google Scholar 

  9. N. H. Stacey, The amelioration of cadmium induced injury in isolated hepatocytes by reduced glutathione,Toxicology 42, 85 (1986).

    Article  PubMed  CAS  Google Scholar 

  10. G. A. Pascoe and D. J. Reed, Cell calcium, vitamin E and thiol redox system in cytotoxicity,Tree Radical & Biol. and Med. 6, 209 (1989).

    Article  CAS  Google Scholar 

  11. M. Sato, K. Yamanobe, and Y. Nagai, Sex related differences in cadmium induced lipid peroxidation in the rat,Life Sci. 33, 903–908 (1983).

    Article  PubMed  CAS  Google Scholar 

  12. S. Gabor, Z. Anca, and E. Bordas, Cadmium induced lipid peroxidation in kidney and testes. Effect of Zinc and Copper,Rev. Roum. Biochim. 15, 113–117 (1978).

    CAS  Google Scholar 

  13. S. V. S. Rana, V. P. Agrawal, and N. G. Bharadwaj, The effects of dietary Cd & Zn on lipids proteins and carbohyderates in rats.Arch. Inds. Hyg. Toxicol. 32, 157–161 (1981).

    CAS  Google Scholar 

  14. Z. A. Placer, L. L. Cushman, and B. C. Johnson, Estimation of the product of lipid peroxidation (malonyl-dialdehyde) in biochemical systems,Analyt. Biochem 16, 359 (1966).

    Article  PubMed  CAS  Google Scholar 

  15. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, Protein measurement with folin phenol reagent,J. Biol. Chem. 193, 265–275 (1951).

    PubMed  CAS  Google Scholar 

  16. G. L. Ellman, Tissue sulphahydryl groups,Arch. Biochem. Biophys. 82, 70–77 (1959).

    Article  PubMed  CAS  Google Scholar 

  17. S. Ohmori, M. IKeda, E. Kasaharae, H. Hyodoh, and K. Hirota, A colorimetric determination of total-glutathione based on its C-terminal glycineresidue and its application to blood liver and yeast,Chem. Pharm. Bul. 29, 1355–1360 (1986).

    Google Scholar 

  18. R. A. Fisher,Statistical Methods for Research Workers, 11th ed., Oliver and Boyd, London (1950).

    Google Scholar 

  19. D. D. Perrin and A. E. Watt, Complex formation of Zinc and Cadmium with glutathione.Biochem. Biophys. Acta 230, 96 (1971).

    PubMed  CAS  Google Scholar 

  20. G. Bellomo, F. Mirabelli, D. Di monte, P. Richelmi, H. Thor, C. Orrenius, and S. Orrenius, Formation and reduction of glutathione mixed disulfides during oxidative stress,Biochem. Pharmacol. 36, 1313 (1987).

    Article  PubMed  CAS  Google Scholar 

  21. N. S. Kosower and E. M. Kosower, Glutathione & Cell membrane thiol status, inFunctions of Glutathione: Biochemical Physiological Toxicological and Clinical Aspects, A. Larrson, S. Orrenius, A. Holmgren, and B. Mannervik, eds., Raven, New York, pp. 307–315 (1983).

    Google Scholar 

  22. P. Graf and H. Sies, Hepatic uptake of cadmium and its biliary release as affected by dithioery thritol and glutathione,Biochem. Pharmacol. 33, 401 (1984).

    Article  Google Scholar 

  23. M. Shimizu and S. Morita, Effects of feeding & fasting on hepatolobular distribution of glutathione and cadmium induced hepato toxicity,Toxicology 75, 97–107 (1992).

    Article  PubMed  CAS  Google Scholar 

  24. S. Horiuchi, M. Inoue, and Y. Morino, γ-glutamyltrans peptidase: Sidedness of its active site on renal brush border membrane,Eur. J. Biochem. 87, 429–437 (1978).

    Article  PubMed  CAS  Google Scholar 

  25. J. S. Cornell and A. Meister, Glutathione and γ-glutamyl cycle enzymes in crypt and villus tip cells of rat jejunal mucosa,Proc. Nat. Acad. Sci. USA 73, 420–422 (1976).

    Article  PubMed  CAS  Google Scholar 

  26. S. V. S. Rana, and P. R. Boora, Antiperoxidative mechanism offered by selenium against liver injury caused by cadmium and mercury in rat.Bull. Env. Cont. Toxicol. 48, 120–124 (1992).

    CAS  Google Scholar 

  27. H. Iwata, T. Masukawa, H. Kitto, and M. Hiyashi, Involvement of tissue sulphydryl in the formation of a complex of methyl mercury with selenium,Biochem. Pharmacol. 30, 3159–3163 (1981).

    Article  PubMed  CAS  Google Scholar 

  28. T. Masukawa, H. Kitto, M. Hayashi, and H. Iwata, Formation and possible role of bis (methyl mercuric) selenide in rats treated with methyl mercury and selenite,Biochem. Pharmacol. 31, 75–78 (1982).

    Article  PubMed  CAS  Google Scholar 

  29. R. Reiter and A. Wendel, Selenium and drugs metabolism III relation of glutathione peroxidase and other hepatic enzyme modulations to dietary supplements,Biochem. Pharmacol. 34, 2287–2290 (1985).

    Article  PubMed  CAS  Google Scholar 

  30. G. S. Shukla, T. Hussain, R. S. Srivastava, and S. V. Chandra, Glutathione peroxidase catalase in liver, kidney, testes and brain regions of rats following cadmium exposure and subsequent withdrawl,Industrial Health 27, 59–69 (1989).

    Article  PubMed  CAS  Google Scholar 

  31. G. W. Burton and K. V. Ingold, Autoxidation of biological molecules. The antioxidant activity of Vitamin E and related chain breaking phènolic antioxidantsin vitro, J. Am. Chem. Soc. 103, 6472 (1981).

    Article  CAS  Google Scholar 

  32. D. C. Liebler, D. S. Kling, and D. J. Reed, Antioxidant protection of phospholipid bilayers by α-tocopherol,J. Biol. Chem. 261, 12,114–12,119 (1986).

    CAS  Google Scholar 

  33. O. Levander, Lead toxicity and nutritional deficiencies,Env. Health Perspect. 29, 115–125 (1979).

    Article  CAS  Google Scholar 

  34. D. Barltrop and H. E. Khoo, Nutritional determinants of lead absorption,Trace Substances in Environmental Health, IX, D. D. Hemphill, ed., University of Missouri Press, Columbia, MO, pp. 369–376 (1975).

    Google Scholar 

  35. D. J. Reed, G. A. Pascoe, and K. Olafsdottir, Some aspects of cell defense mechanism of glutathione and vitamin E during cell injury,Arch. Toxicol. Supp. 11, 34–38 (1987).

    CAS  Google Scholar 

  36. J. Isaacs and F. Binkley, Glutathione dependent control of protein disulfide-sulphydryl content by subcellular fractions of hepatic tissue,Biochem. et Biophys. Acta 497, 192–204 (1977).

    CAS  Google Scholar 

  37. N. S. Kosower and E. M. Kosower, The glutathione status of cells,Intl. Review of Cytology 54, 109–160 (1977).

    Article  Google Scholar 

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  1. Department of Zoology, Ch. Charan Singh University, 250 004, Meerut, India

    S. V. S. Rana & Seema Verma

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  1. S. V. S. Rana
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  2. Seema Verma
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Rana, S.V.S., Verma, S. Protective effects of GSH, vitamin E, and selenium on lipid peroxidation in cadmium-fed rats. Biol Trace Elem Res 51, 161–168 (1996). https://doi.org/10.1007/BF02785435

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  • DOI: https://doi.org/10.1007/BF02785435

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