Skip to main content
SpringerLink
Log in

Ovariectomy increases the level of estrogen receptor mRNA and estrogen receptor binding sites in female rat adipose tissue

  • Published:
Journal of Endocrinological Investigation Aims and scope Submit manuscript

Abstract

The roles of estrogen in the changes in estrogen receptor (ER) mRNA and ER binding sites in rat adipose tissue were studied in female rats. To elucidate the mechanism(s) behind ovariectomy (OVX)-induced obesity, the levels of ER mRNA and ER binding sites in adipose tissue were analyzed three weeks after OVX using Northern blot analysis of ER mRNA and the [3H]E2 binding assay, respectively. OVX induced an increase in body weight, and replacement of estradiol (E2) prevented that increase. Significant increases in the amounts of ER mRNA and in [3H]E2-specific binding were observed after OVX, and E2 replacement reduced both of those increases. These results suggest that E2 may regulate rat obesity directly through ER in adipose tissues.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Wade G.N. Sex hormones, regulatory behaviours, and body weight. In: Rosenblatt J.S., Hinde R.A., Shaw E., Beer C.G. (Eds.), Advances in the Study of Behaviour. Academic Press, New York, 1976, vol. 6, p. 201.

    Google Scholar 

  2. Tarttelin M.F., Gorski R.A. The effects of ovarian steroids on food and water intake and body weight in the female rat. Acta Endocrinol. (Copenh) 72:551, 1973.

    CAS  Google Scholar 

  3. Mook D.G., Kenney N.J., Roberts S., Nussbaum A.I., Rodier W.I. Ovarian-adrenal interactions in regulation of body weight by female rats. J. Comp. Physiol. Psychol. 81:198, 1972.

    Article  PubMed  CAS  Google Scholar 

  4. Wade G.N. Some effects of ovarian hormones on food intake and body weight in female rats. J. Comp. Physiol. Psychol. 88:183, 1975.

    Article  PubMed  CAS  Google Scholar 

  5. Leshner A.I., Collier G. The effects of gonadectomy on the sex differences in dietary self-selection patterns and carcass compositions of rats. Physiol. Behav. 11:671, 1973.

    Article  PubMed  CAS  Google Scholar 

  6. Roy E.J., Wade G.N. Role of food intake in estradiol-induced body weight changes in female rats. Horm. Behav. 8:265, 1977.

    Article  PubMed  CAS  Google Scholar 

  7. Bleau G., Roberts K.D., Chapdelaine A. The in vitro and in vivo uptake and metabolism of steroids in human adipose tissue. J. Clin. Endocrinol. Metab. 39:236, 1974.

    Article  PubMed  CAS  Google Scholar 

  8. Wade G.N., Gray J.M. Cytoplasmic 17\-[3H] estradiol binding in rat adipose tissues. Endocrinology 103:1695, 1978.

    Article  PubMed  CAS  Google Scholar 

  9. Rebuffe-Scrive M. Sex steroid hormones and adipose tissue metabolism in ovariectomized and adrenalectomized rats. Acta. Physiol. Scand. 129:471, 1987.

    PubMed  CAS  Google Scholar 

  10. Wade G.N., Gray J.M. Gonadal effects on food intake and adiposity; a metabolic hypothesis. Physiol. Behav. 22:583, 1979.

    Article  PubMed  CAS  Google Scholar 

  11. Robaire B., Ewing L.L., Irby D.C., Desjardins C. Interactions of testosterone and estradiol- 17\ on the reproductive tract of the male rat. Biol. Reprod. 21:455, 1979.

    Article  PubMed  CAS  Google Scholar 

  12. Ginsburg M., Greenstein B.D., Maclusky N.J., Morris I.D., Thomas P.J. An improved method for the study of high-affinity steroid binding: oestradiol binding in brain and pituitary. Steroids 23: 772, 1974.

    Google Scholar 

  13. Wade G.N., Blaustein J.D. Effects of an antiestrogen on neural estradiol binding and on behaviours in female rats. Endocrinology 102:245, 1978.

    Article  PubMed  CAS  Google Scholar 

  14. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:265, 1951.

    PubMed  CAS  Google Scholar 

  15. Scatchard G. The attractions of proteins for small molecules and ions. Ann. N. Y. Acad. Sci. USA 51:660, 1949.

    Article  CAS  Google Scholar 

  16. Chirgwin J.M., Przybyla A.E., MacDonald R.J., Rutter W.J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294, 1979.

    Article  PubMed  CAS  Google Scholar 

  17. Davis L.G., Dibner M.D., Battey J.F. Basic Methods in Molecular Biology. Elsevier Science Publishing Co., New York, 1986, p. 143.

    Book  Google Scholar 

  18. Koike S., Sakai M., Muramatsu M. Molecular cloning and characterization of rat estrogen receptor cDNA. Nucleic Acids Research 15: 2499, 1987.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  19. Eriksson H.A. Different regulation of the concentration of estrogen receptors in the rat liver and uterus following ovariectomy. FEBS Letter 149:91, 1982.

    Article  CAS  Google Scholar 

  20. Manni A., Baker R., Arafah B.M., Pearson O.H. Uterine oestrogen and progesterone receptors in the ovariectomized rat. J. Endocrinol. 91:281, 1981.

    Article  PubMed  CAS  Google Scholar 

  21. Barley J., Ginsburg M., Greenstein B.D., Maclusky N.J., Thomas P.J. A receptor mediating sexual differentiation? Nature 252: 259, 1974.

    Article  PubMed  CAS  Google Scholar 

  22. Mowles T.F., Ashkallazy B., Mix E. Jr., Sheppard H. Hypothalamic and hypophyseal estradiol-binding complexes. Endocrinology 89: 484, 1971.

    Article  PubMed  CAS  Google Scholar 

  23. Read L.D., Greene G.L., Katzenellenbogen B.S. Regulation of estrogen receptor messenger ribonucleic acid and protein levels in human breast cancer cell lines by sex steroid hormones, their antagonists and grown factors. Mol. Endocrinol. 3:295, 1989.

    Article  PubMed  CAS  Google Scholar 

  24. Saceda M., Lippman M., Chambon P., Lindsey R.L., Ponglikitmongkol M., Puente M., Martin M.B. Regulation of the estrogen receptor in MCF-7 cells by estradiol. Mol. Endocrinol. 2:1157, 1988.

    Article  PubMed  CAS  Google Scholar 

  25. Shupnik M.A., Gordon M.S., Chin W.W. Tissue-specific regulation of rat estrogen receptor mRNAs. Mol. Endocrinol. 3:660, 1989.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Osaka University Medical School, z-z, Yamadaoka, Suita, Osaka, 565, Japan

    Y. Nishikawa,  Hiromasa Ikegami, M. Sakata, T. Mizutani, K. Morishige, H. Kurachi, K. Hirota, A. Miyake & O. Tanizawa

Authors
  1. Y. Nishikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hiromasa Ikegami
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Sakata
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. Mizutani
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K. Morishige
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. H. Kurachi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. K. Hirota
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. A. Miyake
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. O. Tanizawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nishikawa, Y., Ikegami, H., Sakata, M. et al. Ovariectomy increases the level of estrogen receptor mRNA and estrogen receptor binding sites in female rat adipose tissue. J Endocrinol Invest 16, 579–583 (1993). https://doi.org/10.1007/BF03347674

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03347674

Key-words

Search

Navigation