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Differential protein expression in pancreatic islets after treatment with an imidazoline compound

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Abstract.

The effects of an imidazoline compound (BL11282) on protein expression in rat pancreatic islets were investigated with a proteomic approach. The compound increases insulin release selectively at high glucose concentrations and is therefore of interest in type 2 diabetes. Whole cell extracts from isolated drug-treated and native pancreatic rat islets were compared after separation by 2-D gel electrophoresis. Differentially expressed proteins were identified by mass spectrometry; 15 proteins were selectively up-regulated and 7 selectively down-regulated in drug-treated islets. Of special interest among the differentially expressed proteins are those involved in protein folding (Hsp60, protein disulfide isomerase, calreticulin), Ca2+ binding (calgizzarin, calcyclin and annexin I) and metabolism or signalling (pyruvate kinase, alpha enolase and protein kinase C inhibitor 1).

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Authors and Affiliations

  1. Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77, Stockholm, Sweden

    T. Jägerbrink, C. Palmberg, J. Shafqat & H. Jörnvall

  2. Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden

    H. Lexander

  3. The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Stockholm, Sweden

    V. Sharoyko, P.-O. Berggren, S. Efendic & S. Zaitsev

  4. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119992, Russia

    S. Zaitsev

Authors
  1. T. Jägerbrink
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  2. H. Lexander
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  3. C. Palmberg
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  4. J. Shafqat
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  5. V. Sharoyko
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  6. P.-O. Berggren
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  7. S. Efendic
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  8. S. Zaitsev
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  9. H. Jörnvall
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Corresponding author

Correspondence to H. Jörnvall.

Additional information

Received 19 March 2007; received after revision 11 April 2007; accepted 11 April 2007

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Jägerbrink, T., Lexander, H., Palmberg, C. et al. Differential protein expression in pancreatic islets after treatment with an imidazoline compound. Cell. Mol. Life Sci. 64, 1310 (2007). https://doi.org/10.1007/s00018-007-7136-5

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  • DOI: https://doi.org/10.1007/s00018-007-7136-5

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