Abstract
Non-adherent bone marrow-derived cells (NA-BMCs) are a mixed cell population that can give rise to multiple mesenchymal phenotypes and that facilitates hematopoietic recovery. We characterized NA-BMCs by flow cytometry, fibroblast colony-forming units (CFU-f), real-time PCR, and in in vivo experiments. In comparison to adherent cells, NA-BMCs expressed high levels of CD11b+ and CD90+ within the CD45+ cell fraction. CFU-f were significantly declining over the cultivation period, but NA-BMCs were still able to form CFU-f after 5 days. Gene expression analysis of allogeneic NA-BMCs compared to bone marrow (BM) indicates that NA-BMCs contain stromal, mesenchymal, endothelial cells and monocytes, but less osteoid, lymphoid, and erythroid cells, and hematopoietic stem cells. Histopathological data and analysis of weight showed an excellent recovery and organ repair of lethally irradiated mice after NA-BMC transplantation with a normal composition of the BM.
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Abbreviations
- NA-BMCs:
-
Non-adherent bone marrow-derived cells
- CFU-f:
-
Fibroblast colony forming units
- HSC:
-
Hematopoietic stem cells
- TTG mice:
-
Triple trangenic mice
- BMCs:
-
Bone marrow cells
- HSCT:
-
Hematopoietic stem cell transplantation
- GvHD:
-
Graft versus host disease
- MSC:
-
Mesenchymal stem cells
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Acknowledgments
We thank Mr. Jan Matthias Braun, PhD, Translational Centre for Regenerative Medicine, Universität Leipzig and his colleagues for providing and breeding the triple transgenic (TTG) mice, Ms. Stephanie Tuche and Ms. Ramona Blaschke for preparation of the histological slides, Mr. Guido Hildebrandt for preparing irradiation of recipient mice, and Ms. Manuela Ackermann for preparing flow cytometry. The work presented in this paper was funded by the German Federal Ministry of Education and Research (BMBF 0313452, PtJ-Bio, 0313909).
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Fricke, S., Fricke, C., Oelkrug, C. et al. Characterization of murine non-adherent bone marrow cells leading to recovery of endogenous hematopoiesis. Cell. Mol. Life Sci. 67, 4095–4106 (2010). https://doi.org/10.1007/s00018-010-0427-2
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DOI: https://doi.org/10.1007/s00018-010-0427-2