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RETRACTED ARTICLE: Mesenchymal stem cells and neural crest stem cells from adult bone marrow: characterization of their surprising similarities and differences

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This article was retracted on 08 May 2021

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Abstract

The generation of neuronal cells from stem cells obtained from adult bone marrow is of significant clinical interest in order to design new cell therapy protocols for several neurological disorders. The recent identification in adult bone marrow of stem cells derived from the neural crest stem cells (NCSC) might explain the neuronal phenotypic plasticity shown by bone marrow cells. However, little information is available about the nature of these cells compared to mesenchymal stem cells (MSC), including their similarities and differences. In this paper, using transcriptomic as well as proteomic technologies, we compared NCSC to MSC and stromal nestin-positive cells, all of them isolated from adult bone marrow. We demonstrated that the nestin-positive cell population, which was the first to be described as able to differentiate into functional neurons, was a mixed population of NCSC and MSC. More interestingly, we demonstrated that MSC shared with NCSC the same ability to truly differentiate into Tuj1-positive cells when co-cultivated with paraformaldehyde-fixed cerebellar granule neurons. Altogether, those results suggest that both NCSC and MSC can be considered as important tools for cellular therapies in order to replace neurons in various neurological diseases.

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Acknowledgments

This work was supported by a grant from the Fonds National de la Recherche Scientifique (FNRS) of Belgium, by a grant of the Action de Recherche Concertée de la Communauté Française de Belgique, by the Belgian League against Multiple Sclerosis associated with the Leon Fredericq Foundation, and by the Swiss National Science Foundation. P.L. is a senior research associate of the FNRS. A.G. is a Marie Curie Host Fellow for Early Stage Research Training, EURON 020589 within the 6th FP of the EU, Marie Curie Actions - Human Resources and Mobility. Normalization and data filtering were performed using BRB-ArrayTools software version 3.8.1 developed by Dr. Richard Simons and BRB-ArrayTools Development Team http://linus.nci.nih.gov./BRB-ArrayTools.html. Patricia Ernst and Alice Marquet also provided technical assistance to this study. Finally, we would like to thanks Professor Vincent Seutin who gave us the opportunity to use his electrophysiological platform. Finally, we would like to thanks Dr Ormenese and the GIGA plateform of flow cytometry and cell imaging for their valuable advises and technical support.

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Authors and Affiliations

  1. GIGA Neurosciences, University of Liege, CHU B36, 1 Avenue de L’hôpital, 4000, Liège, Belgium

    Sabine Wislet-Gendebien, Emerence Laudet, Virginie Neirinckx, Philippe Alix, Pierre Leprince, Aneta Glejzer & Bernard Rogister

  2. GIGA Research, University of Liege, CHU B34, 1 Avenue de L’hôpital, 4000, Liège, Belgium

    Christophe Poulet

  3. Institute of Anatomy, University of Zurich, 8057, Zurich, Switzerland

    Lukas Sommer & Olga Shakhova

  4. GIGA Development, Stem Cells and Regenerative Medicine, University of Liège, Liège, Belgium

    Bernard Rogister

  5. Department of Neurology, CHU Liège, Liège, Belgium

    Bernard Rogister

  6. GIGA Genomics Platform, University of Liege, CHU B34, 1 Avenue de L’hôpital, 4000, Liège, Belgium

    Benoit Hennuy

Authors
  1. Sabine Wislet-Gendebien
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  2. Emerence Laudet
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  3. Virginie Neirinckx
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  4. Philippe Alix
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  5. Pierre Leprince
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  6. Aneta Glejzer
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  7. Christophe Poulet
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  8. Benoit Hennuy
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  9. Lukas Sommer
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  10. Olga Shakhova
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  11. Bernard Rogister
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Corresponding author

Correspondence to Sabine Wislet-Gendebien.

Additional information

S. Wislet-Gendebien and E. Laudet contributed equally to this work

Electronic supplementary material

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18_2012_937_MOESM1_ESM.tif

Figure S1. Regulated genes between nestin (+) and nestin (-) BMSC: cell fate. Genes expressed by nestin (+) and nestin (-) BMSC have been classified according to their relevance to cell types: stem cells, neural cells, mesenchymal cells, hematopoietic cells, endothelial cells. IPA software was used for classification and gene expression profile analyses. Supplementary material 1 (TIFF 2931 kb)

18_2012_937_MOESM2_ESM.tif

Figure S2. Regulated genes between nestin (+) and nestin (-) BMSC: Signaling pathways. Genes expressed by nestin (+) and nestin (-) BMSC have been classified according to their relevance to signaling pathways. IPA software was used for classification and gene expression profile analyses. Supplementary material 2 (TIFF 2931 kb)

18_2012_937_MOESM3_ESM.tif

Figure S3. Regulated genes between NCSC and MSC clones: cell fate. Genes expressed by NCSC and MSC have been classified according to their relevance to cell types: stem cells, neural cells, mesenchymal cells, hematopoietic cells, endothelial cells. IPA software was used for classification and gene expression profile analyses. Supplementary material 3 (TIFF 2931 kb)

18_2012_937_MOESM4_ESM.tif

Figure S4. Regulated genes between NCSC and MSC clones: Signaling pathways. Genes expressed by NCSC and MSC have been classified according to their relevance to signaling pathways. IPA software was used for classification and gene expression profile analyses. Supplementary material 4 (TIFF 2931 kb)

18_2012_937_MOESM5_ESM.pdf

Table S1. List of regulated genes in nestin (+) BMSC compared to nestin (-) BMSC. 379 genes have been identified as differentially expressed between nestin (+) and nestin (-) BMSC, with a minimum of twofold difference. Supplementary material 5 (PDF 93 kb)

Supplementary material 6 (XLS 23 kb)

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Wislet-Gendebien, S., Laudet, E., Neirinckx, V. et al. RETRACTED ARTICLE: Mesenchymal stem cells and neural crest stem cells from adult bone marrow: characterization of their surprising similarities and differences. Cell. Mol. Life Sci. 69, 2593–2608 (2012). https://doi.org/10.1007/s00018-012-0937-1

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  • DOI: https://doi.org/10.1007/s00018-012-0937-1

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