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Lack of kinin B1 receptor potentiates leptin action in the liver

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Abstract

Kinins B1 and B2 receptors (B1R and B2R) are classically associated with inflammation, but our group has recently demonstrated new roles for B1R in metabolism using a knockout model (B1 −/−). B1 −/− mice display improvement on leptin and insulin sensitivity and is protected from high fat diet (HFD)-induced obesity. Here, we evaluate the hepatic effects of the B1R ablation and its role on hepatic function. Despite no expression of hepatic B1R, HFD-induced hepatic lipid accumulation was lower than in control animals. B1 −/− mice also presented lower hepatic lipogenesis and SCD1 protein content in the liver. When stimulated with exogenous leptin, B1 −/− mice exhibited increased hepatic pJAK2. Similarly, leptin signaling was enhanced in the liver of ob/ob–B1 −/− mice, as demonstrated by increased levels of pSTAT3 compared to ob/ob. Plasma concentrations of intercellular adhesion molecule 1, fetuin A, leukemia inhibitory factor, tissue inhibitor of metalloprotease-1, resistin, and oncostatin M were reduced in B1 −/−. Finally, B1 −/− mice have increased gene expression of hepatic B2 receptor, but no difference in leptin receptor expression. Our results show that B1 −/− mice are protected from non-alcoholic fatty liver disease (NAFLD) after HFD treatment. Since B1R expression was not observed in the liver after HFD, we propose that the cross talk between the adipose tissue and the liver, mainly through leptin, is an important factor contributing to the observed results. Besides that, several other inflammatory mediators already correlated with NAFLD or liver function were found to be altered in our model. Taken together, our data suggest that B1R plays an important role in hepatic steatosis development.

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Acknowledgments

This work was supported by grants of Fapesp Processo 2008/06676-8, CNPq, and CAPES.

Conflict of interest

No potential conflicts of interest relevant to this article were reported.

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Authors and Affiliations

  1. Department of Biophysics, Federal University of São Paulo—UNIFESP/EPM, Rua Pedro de Toledo, 669, 9° andar, Vila Clementino, 04039-032, São Paulo, São Paulo, Brazil

    Raphael Gomes Fonseca, Vicencia Micheline Sales, Carlos Castilho Barros, Ronaldo Carvalho Araújo & João Bosco Pesquero

  2. School of Applied Science, State University of Campinas—UNICAMP, Limeira, São Paulo, Brazil

    Eduardo Ropelle

  3. Department of Physiology, Federal University of São Paulo—UNIFESP/EPM, São Paulo, São Paulo, Brazil

    Lila Oyama

  4. Department of Pathology, Federal University of São Paulo—UNIFESP/EPM, São Paulo, São Paulo, Brazil

    Silvia Saiuli Iuki Ihara

  5. Department of Internal Medicine, State University of Campinas—UNICAMP, Campinas, São Paulo, Brazil

    Mário Jose Abdalla Saad

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  1. Raphael Gomes Fonseca
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  2. Vicencia Micheline Sales
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  8. Ronaldo Carvalho Araújo
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  9. João Bosco Pesquero
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Correspondence to João Bosco Pesquero.

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Raphael Gomes Fonseca and Vicencia Micheline Sales contributed equally to this work.

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Fonseca, R.G., Sales, V.M., Ropelle, E. et al. Lack of kinin B1 receptor potentiates leptin action in the liver. J Mol Med 91, 851–860 (2013). https://doi.org/10.1007/s00109-013-1004-6

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  • DOI: https://doi.org/10.1007/s00109-013-1004-6

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