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Monolithic spin column extraction and GC-MS for the simultaneous assay of diquat, paraquat, and fenitrothion in human serum and urine

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Abstract

We present a method based on monolitic spin column extraction and gas chromatography–mass spectrometry as an analytical method for screening diquat (DQ), paraquat (PQ), and fenitrothion in serum and urine. This method is useful for clinical and forensic toxicological analyses. Recovery of DQ, PQ, and fenitrothion from serum and urine, spiked at concentrations between 0.1, 2.5, 20, and 45 μg/ml, ranged from 51.3% to 106.1%. Relative standard deviation percentages were between 3.3% and 14.8%. Detection and quantitation limits for serum and urine were 0.025 and 0.05 μg/ml, respectively, for DQ, 0.1 and 0.1 μg/ml, respectively, for PQ, and 0.025 and 0.05 μg/ml, respectively, for fenitrothion. Therefore, these compounds can be detected and quantified in the case of acute poisoning.

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Acknowledgments

This work was supported in part by a Grant-in-Aid for Scientific Research (C) (No. 21590745) from the Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. Department of Emergency and Critical Care Medicine, Tokai University School of Medicine, Shimokasuya 143, Isehara, Kanagawa, 259-1143, Japan

    Takeshi Saito, Tomokazu Fukushima & Sadaki Inokuchi

  2. GL Sciences Inc, Sayamagahara, Iruma, Saitama, 358-0032, Japan

    Yuko Yui & Shota Miyazaki

  3. Scientific Investigation Laboratory, Hiroshima Prefectural Police Headquarters, Kohnan 2-26-3, Naka-ku, Hiroshima, 730-0825, Japan

    Akihiro Nakamoto

  4. Department of Forensic Medicine, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima, 734-8551, Japan

    Akihiro Nakamoto & Akira Namera

Authors
  1. Takeshi Saito
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  2. Tomokazu Fukushima
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  3. Yuko Yui
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  4. Shota Miyazaki
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  5. Akihiro Nakamoto
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  6. Akira Namera
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  7. Sadaki Inokuchi
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Corresponding author

Correspondence to Takeshi Saito.

Additional information

Published in the special issue Forensic Toxicology with Guest Editors Frank T. Peters, Hans H. Maurer, and Frank Musshoff.

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Saito, T., Fukushima, T., Yui, Y. et al. Monolithic spin column extraction and GC-MS for the simultaneous assay of diquat, paraquat, and fenitrothion in human serum and urine. Anal Bioanal Chem 400, 25–31 (2011). https://doi.org/10.1007/s00216-010-4633-4

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  • DOI: https://doi.org/10.1007/s00216-010-4633-4

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