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Sensitive and rapid amperometric magnetoimmunosensor for the determination of Staphylococcus aureus

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Abstract

The preparation and characteristics of a disposable amperometric magnetoimmunosensor, based on the use of functionalized magnetic beads (MBs) and gold screen-printed electrodes (Au/SPEs), for the specific detection and quantification of Staphylococcal protein A (ProtA) and Staphylococcus aureus (S. aureus) is reported. An antiProtA antibody was immobilized onto ProtA-modified MBs, and a competitive immunoassay involving ProtA antigen labelled with HRP was performed. The resulting modified MBs were captured by a magnetic field on the surface of tetrathiafulvalene-modified Au/SPEs and the amperometric response obtained at −0.15 V vs the silver pseudo-reference electrode of the Au/SPEs after the addition of H2O2 was used as transduction signal. The developed methodology showed very low limits of detection (1 cfu S. aureus/mL of raw milk samples), and a good selectivity against the most commonly involved foodborne pathogens originating from milk. These features, together with a short analysis time (2 h), the simplicity, and easy automation and miniaturization of the required instrumentation make the developed methodology a promising alternative in the development of devices for on-site analysis.

Schematic display of the developed S. aureus amperometric magnetoimmunosensor

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Acknowledgements

The financial support of the Spanish Ministerio de Ciencia e Innovación Research Project CTQ2009-09351BQU, and the AVANSENS Program from the Comunidad de Madrid (S2009PPQ-1642) are gratefully acknowledged. B. Esteban-Fernández de Ávila acknowledges a FPI fellowship from the Spanish Ministerio de Ciencia e Innovación.

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Authors and Affiliations

  1. Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, 28040, Madrid, Spain

    Berta Esteban-Fernández de Ávila, María Pedrero, Susana Campuzano, Vanessa Escamilla-Gómez & José M. Pingarrón

Authors
  1. Berta Esteban-Fernández de Ávila
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  2. María Pedrero
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  3. Susana Campuzano
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  4. Vanessa Escamilla-Gómez
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  5. José M. Pingarrón
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Corresponding author

Correspondence to José M. Pingarrón.

Additional information

Published in the special issue Euroanalysis XVI (The European Conference on Analytical Chemistry) with guest editor Slavica Ražić.

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Esteban-Fernández de Ávila, B., Pedrero, M., Campuzano, S. et al. Sensitive and rapid amperometric magnetoimmunosensor for the determination of Staphylococcus aureus . Anal Bioanal Chem 403, 917–925 (2012). https://doi.org/10.1007/s00216-012-5738-8

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  • DOI: https://doi.org/10.1007/s00216-012-5738-8

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