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Solid-phase extraction for HPLC analysis of dietary folates

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Abstract

Phenyl and cyclohexyl bonded silicas used for the solid-phase extraction (SPE) of dietary folates were studied and compared in terms of their retention capacities and recoveries. The SPE procedure was optimised to achieve the maximum recovery. The stabilities of folates in elution buffers and during the SPE procedure were also studied. The use of ascorbic acid throughout the SPE procedure was found to be important for the stabilisation of tetrahydrofolate – the most labile folate compound, whereas the use of both ascorbic acid and 2-mercaptoethanol was necessary to stabilise tetrahydrofolate during the storage of the eluates in the autosampler until analysis. Two purification methods, using phenyl endcapped (PH EC) cartridges or the combination of strong anion-exchange (SAX) and PH EC cartridges, were applied to the determination of folates in different food extracts, and verified by correlation with the usual purification method that uses SAX cartridges. The combination of SAX and PH EC cartridges was found to give the cleanest extracts.

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Acknowledgements

We are very grateful to Professor Margaretha Jägerstad for her interest in this study and for valuable discussions on the manuscript. This work was financially supported by The Swedish Board for Technological and Industrial Development (NUTEK) and three food companies: Procordia Food AB, Cerealia AB and Arla Foods. The financial sponsorship of Dr. Elena Yazynina by The Swedish Institute is also gratefully acknowledged.

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Authors and Affiliations

  1. Department of Food Science, Swedish University of Agricultural Sciences, P.O. Box 7051, 75007, Uppsala, Sweden

    Charlotte Nilsson, Madelene Johansson, Lena Strålsjö & Jelena Jastrebova

  2. Institute of Biochemistry, Russian Academy of Sciences, Leninsky Prospect 33, 119071, Moscow, Russia

    Elena Yazynina

Authors
  1. Charlotte Nilsson
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  2. Madelene Johansson
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  3. Elena Yazynina
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  4. Lena Strålsjö
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  5. Jelena Jastrebova
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Correspondence to Jelena Jastrebova.

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Nilsson, C., Johansson, M., Yazynina, E. et al. Solid-phase extraction for HPLC analysis of dietary folates. Eur Food Res Technol 219, 199–204 (2004). https://doi.org/10.1007/s00217-004-0941-2

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  • DOI: https://doi.org/10.1007/s00217-004-0941-2

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