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Thermodynamic interactions of a cis and trans benzanilide with Escherichia coli bacterial membranes

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Abstract

The activity of a membrane interactive cis and trans benzanilide against Escherichia coli membrane mimics was investigated using Langmuir monolayers. It was found that in the presence of E. coli lipid mix monolayers, cis-benzanilide induced maximal surface pressure changes of 1 mN m−1, whereas a reduced interaction was observed with trans-benzanilide. Compression isotherm analysis of these monolayers showed ∆G mix < 0, indicating membrane stabilisation by both compounds. The binding coefficient for trans-benzanilide was circa 890 μM and for the cis-benzanilide was 820 μM, suggesting that both benzanilide compounds bound to the E. coli membranes.

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Acknowledgments

The authors thank Prof. LHG Morton, University of Central Lancashire, for support with the antimicrobial assay.

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Authors and Affiliations

  1. School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston, PR1 2HE, UK

    Sarah R. Dennison & Timothy J. Snape

  2. Office of the Vice Chancellor, University of Central Lancashire, Preston, PR1 2HE, UK

    David A. Phoenix

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  1. Sarah R. Dennison
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  2. Timothy J. Snape
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  3. David A. Phoenix
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Correspondence to David A. Phoenix.

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Dennison, S.R., Snape, T.J. & Phoenix, D.A. Thermodynamic interactions of a cis and trans benzanilide with Escherichia coli bacterial membranes. Eur Biophys J 41, 687–693 (2012). https://doi.org/10.1007/s00249-012-0835-3

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  • DOI: https://doi.org/10.1007/s00249-012-0835-3

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