Abstract
Biofilm-related infections have become a major clinical concern. Typically, animal models that involve inoculation with planktonic bacteria have been used to create positive infection signals and examine antimicrobial strategies for eradicating or preventing biofilm-related infection. However, it is estimated that 99.9% of bacteria in nature dwell in established biofilms. As such, open wounds have significant potential to become contaminated with bacteria that reside in a well-established biofilm. In this study, a modified CDC biofilm reactor was developed to repeatably grow mature biofilms of Staphylococcus aureus on the surface of polyetheretherketone (PEEK) membranes for inoculation in a future animal model of orthopaedic implant biofilm-related infection. Results indicated that uniform, mature biofilms repeatably grew on the surface of the PEEK membranes.
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Acknowledgments
This material is based upon study supported by the Office of Research and Development, Rehabilitation R&D Service, Department of Veterans Affairs, Salt Lake City, UT. The project described was also supported by Award Number R01AR057185 from the National Institute Of Arthritis And Musculoskeletal And Skin Diseases. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute Of Arthritis And Musculoskeletal And Skin Diseases or the National Institutes of Health. This study was also supported by the Albert and Margaret Hofmann Chair and the Department of Orthopaedics, University of Utah School of Medicine, Salt Lake City, UT. The authors also acknowledge the help of Brad Isaacson, PhD for his contributions to the design of the modified CDC biofilm reactor and Dennis Romney for machining the modified reactor parts.
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Williams, D.L., Woodbury, K.L., Haymond, B.S. et al. A Modified CDC Biofilm Reactor to Produce Mature Biofilms on the Surface of PEEK Membranes for an In Vivo Animal Model Application. Curr Microbiol 62, 1657–1663 (2011). https://doi.org/10.1007/s00284-011-9908-2
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DOI: https://doi.org/10.1007/s00284-011-9908-2