Abstract
Suspected colonies of methicillin-resistant Staphylococcus aureus (MRSA) on chromogenic, MRSASelect (BioRad) medium were confirmed using routine microbiological methods, and a multiplex real-time PCR (n = 108). Although the specificity of MRSASelect assessed at 24 h of incubation was much higher than that of 48 h (91.4 vs. 60 %), extending the incubation time to 48 h, along with PCR confirmation, increased the total number of true positive samples by 27.8 %. These results provide a cost effective method for sensitive and specific detection of MRSA.
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Ben Nsira S, Dupuis M, Leclercq R (2006) Evaluation of MRSA Select, a new chromogenic medium for the detection of nasal carriage of methicillin-resistant Staphylococcus aureus. Int J Antimicrob Agents 27:561–564
Boyce JM, Havill NL, Kohan C, Dumigan DG, Ligi CE (2004) Do infection control measures work for methicillin-resistant Staphylococcus aureus? Infect Control Hosp Epidemiol 25:395–401
Clinical and Laboratory Standards Institute (2011) Performance standards for antimicrobial susceptibility testing: 22nd informational supplement. M100-S22. CLSI, Wayne
Delaney JA, Schneider-Lindner V, Brassard P, Suissa S (2008) Mortality after infection with methicillin-resistant Staphylococcus aureus (MRSA) diagnosed in the community. BMC Med 6:2
Grundmann H, Aires-de-Sousa M, Boyce J, Tiemersma E (2006) Emergence and resurgence of methicillin-resistant Staphylococcus aureus as a public-health threat. Lancet 368:874–885
Harbarth S, Martin Y, Rohner P, Henry N, Auckenthaler R, Pittet D (2000) Effect of delayed infection control measures on a hospital outbreak of methicillin-resistant Staphylococcus aureus. J Hosp Infect 46:43–49
Hubben G, Bootsma M, Luteijn M, Glynn D, Bishai D, Bonten M, Postma M (2011) Modelling the costs and effects of selective and universal hospital admission screening for methicillin-resistant Staphylococcus aureus. PLoS One 6(3):e14783. doi:10.1371/journal.pone.0014783
Jernigan JA, Titus MG, Groschel DH, Getchell-White S, Farr BM (1996) Effectiveness of contact isolation during a hospital outbreak of methicillin-resistant Staphylococcus aureus. Am J Epidemiol 143:496–504
Louie L, Soares D, Meaney H, Vearncombe M, Simor AE (2006) Evaluation of a new chromogenic medium, MRSA select, for detection of methicillin-resistant Staphylococcus aureus. J Clin Microbiol 44:4561–4563
Lucet JC, Chevret S, Durand-Zaleski I, Chastang C, Regnier B (2003) Prevalence and risk factors for carriage of methicillin-resistant Staphylococcus aureus at admission to the intensive care unit: results of a multicenter study. Arch Intern Med 163:181–188
Luteijn JM, Hubben GAA, Pechlivanoglou P, Bonten MJ, Postma MJ (2010) Diagnostic accuracy of culture-based and PCR-based detection tests for methicillin-resistant Staphylococcus aureus: a meta-analysis. Clin Microbiol Infect 17:146–154
Marlowe EM, Bankowski MJ (2011) Conventional and molecular methods for the detection of methicillin-resistant Staphylococcus aureus. J Clin Microbiol 49:S53–S56
Nahimana I, Francioli P, Blanc DS (2006) Evaluation of three chromogenic media (MRSA-ID, MRSA-Select and CHROMagar MRSA) and ORSAB for surveillance cultures of methicillin-resistant Staphylococcus aureus. Clin Microbiol Infect 12:1168–1174
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Hasan, M.R., Brunstein, J.D., Al-Rawahi, G. et al. Optimal Use of MRSASelect and PCR to Maximize Sensitivity and Specificity of MRSA Detection. Curr Microbiol 66, 61–63 (2013). https://doi.org/10.1007/s00284-012-0241-1
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DOI: https://doi.org/10.1007/s00284-012-0241-1