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Immunolocalization of protein 4.1B/DAL-1 during neoplastic transformation of mouse and human intestinal epithelium

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Abstract

Recently, we have reported that the protein 4.1B immunolocalization occurred only in matured columnar epithelial cells of normal rat intestines. This finding suggested that protein 4.1B expression could be examined for a possible change during neoplastic transformation of the intestinal mucosa. In the present study, we first present the distribution of mouse protein 4.1B in normal intestinal epithelial cells and tumor cells using the adenomatous polyposis coli (Apc) mutant mouse model. A low level of protein 4.1B expression coincided with the phenotypic transition to carcinoma. To examine the protein 4.1B expression in human intestinal mucosa, we used another antibody against an isoform of the human protein 4.1B, DAL-1 (differentially expressed adenocarcinoma of the lung). Human DAL-1 was also expressed in matured epithelial cells in human colons, with a definite expression gradient along the crypt axis. In human colorectal cancer cells, however, DAL-1 expression was not detected. These results suggest that mouse protein 4.1B and human DAL-1 might have a striking analogy of functions, which may be integrally involved in epithelial proliferation. We propose that loss of protein 4.1B/DAL-1 expression might be a marker of intestinal tumors, indicative of a tumor suppressor function in the intestinal mucosa.

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Acknowledgements

The authors would like to thank Dr. Riccardo Fodde, Department of Pathology, Josephone Nefkens Institute, Erasmus University Medical Center, for sending us the intestinal tissues of Apc mutant model mouse, Apc+/Apc1638 N. The authors also thank Drs. Takeshi Baba, Yasuhisa Fujii, and Zagreb Zea-Aragon, Department of Anatomy, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, for their constructive comments on this work.

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Authors and Affiliations

  1. Department of Anatomy, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Tamaho, 409-3898, Yamanashi, Japan

    Nobuhiko Ohno, Nobuo Terada & Shinichi Ohno

  2. Department of Pathology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Tamaho, 409-3898, Yamanashi, Japan

    Shin-ichi Murata & Ryohei Katoh

  3. Department of Human Gene Research, Kazusa DNA Research Institute, Kisarazu, Japan

    Hisashi Yamakawa & Osamu Ohara

  4. Department of Neurosurgery & Hermelin Brain Tumor Center, Henry Ford Hospital, Detroit, USA

    Irene F. Newsham

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  1. Nobuhiko Ohno
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  2. Nobuo Terada
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  3. Shin-ichi Murata
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  4. Hisashi Yamakawa
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  7. Osamu Ohara
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  8. Shinichi Ohno
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Correspondence to Shinichi Ohno.

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Ohno, N., Terada, N., Murata, Si. et al. Immunolocalization of protein 4.1B/DAL-1 during neoplastic transformation of mouse and human intestinal epithelium. Histochem Cell Biol 122, 579–586 (2004). https://doi.org/10.1007/s00418-004-0716-7

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