Skip to main content
SpringerLink
Log in

Helicase-like transcription factor exhibits increased expression and altered intracellular distribution during tumor progression in hypopharyngeal and laryngeal squamous cell carcinomas

  • Original Article
  • Published:
Virchows Archiv Aims and scope Submit manuscript

Abstract

The helicase-like transcription factor (HLTF) belongs to the SWI/SNF family of proteins that use the energy from adenosine triphosphate hydrolysis to remodel chromatin during a variety of cellular processes. HLTF is also involved in DNA repair. Using computer-assisted microscopy, the immunohistochemical expression of HLTF was determined using a series of 100 hypopharyngeal and 56 laryngeal squamous cell carcinomas (SCCs) compared to tumor-free epithelia (60 cases) and dysplasias (92 cases). In hypopharyngeal SCC tumor progression, increased HLTF expression was associated with the percentage of immunopositive epithelial tissue areas (p = 0.02) and the staining intensity of the positive area (p = 0.0005). In the cases of laryngeal lesions, the immunolabeling intensity of HLTF significantly decreased with malignancy (p = 0.01). We also observed a significant shift of HLTF expression from the cytoplasm toward the nuclear compartment (p = 0.0007). Our data reveal an association between the presence of HLTF and neoplastic progression of hypopharyngeal and laryngeal SCCs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Debauve G, Capouillez A, Belayew A et al (2008) HLTF and its implication in cancer progression. Cell Mol Life Sci 65:591–604

    Article  PubMed  CAS  Google Scholar 

  2. Moinova HR, Chen WD, Shen L (2002) HLTF gene silencing in human colon cancer. Proc Natl Acad Sci USA 99:4562–4567

    Article  PubMed  CAS  Google Scholar 

  3. Versteege I, Sevenet N, Lange J et al (1998) Truncating mutations of hSNF5/INI1 in aggressive paediatric cancer. Nature 394:203–206

    Article  PubMed  CAS  Google Scholar 

  4. Hibi K, Kodera Y, Ito K et al (2005) Aberrant methylation of HLTF, SOCS-1, and CDH13 genes is shown in colorectal cancers without lymph node metastasis. Dis Colon Rectum 48:1282–1286

    Article  PubMed  Google Scholar 

  5. Hibi K, Nakao A (2006) Highly-methylated colorectal cancers show poorly-differentiated phenotype. Anticancer Res 26:4263–4266

    PubMed  CAS  Google Scholar 

  6. Hibi K, Nakao A (2006) Lymph node metastasis is infrequent in patients with highly-methylated colorectal cancer. Anticancer Res 26:55–58

    PubMed  CAS  Google Scholar 

  7. Hibi K, Nakayama H, Kanyama Y et al (2003) Methylation pattern of HLTF gene in digestive tract cancers. Int J Cancer 104:433–436

    Article  PubMed  CAS  Google Scholar 

  8. Kim YH, Petko Z, Dzieciatkowski S et al (2006) CpG island methylation of genes accumulates during the adenoma progression step of the multistep pathogenesis of colorectal cancer. Genes Chromosomes Cancer 45:781–789

    Article  PubMed  CAS  Google Scholar 

  9. Hamai Y, Oue N, Mitani Y et al (2003) DNA hypermethylation and histone hypoacetylation of the HLTF gene are associated with reduced expression in gastric carcinoma. Cancer Sci 94:692–698

    Article  PubMed  CAS  Google Scholar 

  10. Kim JJ, Chung SW, Kim JH et al (2006) Promoter methylation of helicase-like transcription factor is associated with the early stages of gastric cancer with family history. Ann Oncol 17:657–662

    Article  PubMed  CAS  Google Scholar 

  11. Leung WK, Yu J, Bai AH (2003) Inactivation of helicase-like transcription factor by promoter hypermethylation in human gastric cancer. Mol Carcinog 37:91–97

    Article  PubMed  CAS  Google Scholar 

  12. Oue N, Mitani Y, Motoshita J et al (2006) Accumulation of DNA methylation is associated with tumor stage in gastric cancer. Cancer 106:1250–1259

    Article  PubMed  CAS  Google Scholar 

  13. Fukuoka T, Hibi K, Nakao A (2006) Aberrant methylation is frequently observed in advanced esophageal squamous cell carcinoma. Anticancer Res 26:3333–3335

    PubMed  CAS  Google Scholar 

  14. Kang S, Kim JW, Kang GH et al (2006) Comparison of DNA hypermethylation patterns in different types of uterine cancer: cervical squamous cell carcinoma, cervical adenocarcinoma and endometrial adenocarcinoma. Int J Cancer 118:2168–2171

    Article  PubMed  CAS  Google Scholar 

  15. Debauve G, Nonclercq D, Ribaucour F et al (2006) Early expression of the helicase-like transcription factor (HLTF/SMARCA3) in an experimental model of estrogen-induced renal carcinogenesis. Mol Cancer 5:23

    Article  PubMed  CAS  Google Scholar 

  16. Hyams VJ, Batsakis JG and Michaels L eds. (1998) Tumors of the upper respiratory tract and ear. In: Atlas of Tumor Pathology, Armed Forces Institute of Pathology, Washington D.C.

  17. Wittekind C, Greene FL, Hutter RRP et al (2004) TNM Atlas, 5th edn. UICC Springer, Berlin

    Google Scholar 

  18. Ding H, Descheemaeker K, Marynen P et al (1996) Characterization of a helicase-like transcription factor involved in the expression of the human plasminogen activator inhibitor-1 gene. DNA Cell Biol 15:429–442

    Article  PubMed  CAS  Google Scholar 

  19. Saussez S, Lorfevre F, Lequeux T et al (2008) The determination of the levels of circulating galectin-1 and -3 in HNSCC patients could be used to monitor tumor progression and/or responses to therapy. Oral Oncology 44:86–93

    Article  PubMed  CAS  Google Scholar 

  20. Galle N, Pilch Z, Sidransky D et al (2006) Head and neck tumours. IARC, Lyon, France

    Google Scholar 

  21. Saussez S, Decaestecker C, Lorfevre F et al (2008) Increased expression and altered intracellular distribution of adhesion/growth-regulatory lectins galectins-1 and -7 during tumour progression in hypopharyngeal and laryngeal squamous cell carcinomas. Histopathology 52:483–493

    Article  PubMed  CAS  Google Scholar 

  22. Unk I, Hajdú I, Fátyol K et al (2008) HLTF functions as a ubiquitin ligase for proliferating cell nuclear antigen polyubiquitination. Proc Natl Acad Sci USA 105:3768–3773

    Article  PubMed  CAS  Google Scholar 

  23. Gong X, Kaushal S, Ceccarelli E et al (1997) Developmental regulation of Zbu1, a DNA-binding member of the SWI2/SNF2 family. Dev Biol 183:166–182

    Article  PubMed  CAS  Google Scholar 

  24. Mansharamani M, Hewetson A, Chilton BS (2001) Cloning and characterization of an atypical type IV P-type ATPase that binds to the RING motif of RUSH transcription factors. J Biol Chem 276:3641–3649

    Article  PubMed  CAS  Google Scholar 

  25. Shah J (2003) Head and neck surgery and oncology. Mosby, London

    Google Scholar 

  26. Roland NJ, Caslin AW, Nash J et al (1992) Value of grading squamous cell carcinoma of the head and neck. Head Neck 14:224–229

    Article  PubMed  CAS  Google Scholar 

  27. Audet N, Beasly NJ, Mac Millan C et al (2005) Lymphatic vessel density, nodal metastases and prognosis in patients with head and neck cancer. Arch Otolaryngol Head Neck Surg 131:1065–1070

    Article  PubMed  Google Scholar 

  28. Saussez S, Cucu R, Decaesteker C et al (2006) Galectin-7 (p53-induced gene-1): a new prognostic predictor of recurrence and survival in stage IV hypopharyngeal cancer. Ann Surg Oncol 13:999–1009

    Article  PubMed  Google Scholar 

  29. Saussez S, Lorfebvre F, Cucu D et al (2007) High level of galectin-1 expression is a negative prognostic predictor of recurrence in laryngeal squamous cell carcinomas. Int J Oncol 30:1109–1117

    PubMed  CAS  Google Scholar 

Download references

Acknowledgements

We thank the Fondation pour la Recherche Médicale dans le Hainaut (Hainaut, Belgium) for funding (S. Saussez and A. Belayew). We are also grateful to G. Ninfa for expert technical assistance.

Conflicts of interest

The authors declare no conflict of interest relevant to this article.

Author information

Authors and Affiliations

  1. Laboratory of Anatomy, Faculty of Medicine and Pharmacy, University of Mons-Hainaut, Pentagone 1B-Avenue du Champ de Mars, 6, 7000, Mons, Belgium

    Aurélie Capouillez, Olivier Filleul & Sven Saussez

  2. Laboratory of Molecular Biology, Faculty of Medicine and Pharmacy, University of Mons-Hainaut, Mons, Belgium

    Aurélie Capouillez, Frederique Coppée & Alexandra Belayew

  3. Laboratory of Toxicology, Institute of Pharmacy, Université Libre de Bruxelles (ULB), Brussels, Belgium

    Christine Decaestecker

  4. Department of Oto-Rhino-Laryngology, Faculty of Medicine, Hôpital Claude Huriez and Centre de Biologie-Pathologie-CHRU, Lille, France

    Dominique Chevalier

  5. Department of Pathology, Faculty of Medicine, Hôpital Claude Huriez and Centre de Biologie-Pathologie-CHRU, Lille, France

    Xavier Leroy

Authors
  1. Aurélie Capouillez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christine Decaestecker
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Olivier Filleul
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dominique Chevalier
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Frederique Coppée
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xavier Leroy
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Alexandra Belayew
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Sven Saussez
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sven Saussez.

Additional information

Christine Decaestecker is a Senior Research Associate of the Belgian National Fund for Scientific Research. A. Capouillez held pre-doctoral fellowships from the Fonds de Recherche pour l’ Industrie et l’ Agriculture (FRIA, Belgium).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Capouillez, A., Decaestecker, C., Filleul, O. et al. Helicase-like transcription factor exhibits increased expression and altered intracellular distribution during tumor progression in hypopharyngeal and laryngeal squamous cell carcinomas. Virchows Arch 453, 491–499 (2008). https://doi.org/10.1007/s00428-008-0675-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00428-008-0675-9

Keywords

Search

Navigation