Skip to main content
Log in

Comparative efficacy of Annona squamosa and Azadirachta indica extracts against Boophilus microplus Izatnagar isolate

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

In the search of developing herbal acaricides, eight medicinal plants were screened for their efficacy against Boophilus microplus, the widely distributed tick species in India. Of the seven extracts screened, the extracts prepared from the Annona squamosa seed showed very high level of efficacy (70.8%) after 24 h of treatment. The effect of treatment on oviposition of the survived ticks was also assessed, and a significant reduction (P < 0.05) in the reproductive index was noted in comparison to control. When efficacy of the in vitro optimized concentration of A. squamosa was compared with previously tested extract of Azadirachta indica in in vivo model, it was observed that the extracts prepared from A. indica is more efficacious than the extracts of A. squamosa. A comparable efficacy against B. microplus fed on animals treated with herbal extracts and commonly used synthetic acaricide was noted. The possibility of using the herbal extracts in IPM format for the management of ticks is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Benavides OE, Hernandez MG, Castro RNA, AH RBJL (2001) Preliminary evaluation of neem (Azadirachta indica) extracts as alternative for cattle ticks Boophilus microplus. Riv Columb Entomol 27:1–8

    Google Scholar 

  • Bicalho KA, Ferreire F, Borges LMF, Ribeiro MFB (2001) In vitro evaluation of the effects of some acaricides on life stages of Rhipicephalus sanguineus (Acari: Ixodidae). Arq Bras Med Vet Zootec 53:548–552

    Article  Google Scholar 

  • Chungsamarnyart NS, Jiwajinda W, Jansawan U, Buranasilpin KP (1988) Effective plant crude-extracts on the ticks (Boophilus microplus). I. Larvicidal Action. Kasetsart J Nat Sci Suppl 22:37–41

    Google Scholar 

  • FAO (2004) Resistance management and integrated parasite control in ruminants. Guidelines. Animal Production and Health Division, pp 25–77

  • Finney DJ (1962) Probit analysis—a statistical treatment of the sigmoid response curve. Cambridge University Press, Cambridge, pp 1–318

    Google Scholar 

  • Ghosh S, Azhahianambi P (2007) Laboratory rearing of Theileria annulata-free Hyalomma anatolicum anatolicum ticks. Exp Appl Acarol 43:137–146

    Article  PubMed  CAS  Google Scholar 

  • Ghosh S, Singh NK, Das G (2005) Assessment of duration of immunity in calves immunized by glycoproteins isolated from the larvae of Hyalomma anatolicum anatolicum and Boophilus microplus. Parasitol Res 95:319–326

    Article  PubMed  CAS  Google Scholar 

  • Ghosh S, Azhahianambi P, De la Fuente J (2006) Control of ticks of ruminants with special emphasis on livestock farming system in India—present and future possibilities for integrated control: a review. Exp Appl Acarol 40:49–66

    Article  PubMed  CAS  Google Scholar 

  • Graf JF, Gogolewski R, Leach BN (2004) Tick control: an industry point of view. Parasitology 129:S247–S442

    Article  Google Scholar 

  • Hart RJ (1986) Mode of action of agent used against arthropod parasites. Chemotherapy of parasitic diseases. Plenum, New York, pp 585–601

    Google Scholar 

  • Kalakumar B, Kumar HSA, Kumar BA, Reddy KS (2000) Evaluation of custard seed oil and neem oil as acaricides. J Vet Parasitol 14:171–172

    Google Scholar 

  • Khan MH, Naithani RC, Singh (kr) S (1982) Study on the bionomics of Nosoma monostrosum (Nuttall and Warburton) (Acari: Ixodidae). Acarologia 23:119–123

    Google Scholar 

  • Minjauw B, Mc Leod A (2003) Tick borne diseases and poverty. The impact of tick and tick borne diseases on the livelihoods of small-scale and marginal livestock owners in India and eastern and southern Africa. Research report, DFID Animal Health Programme, Centre for Tropical Veterinary Medicine, University of Edinburgh, UK

  • Narang C, Suganya M, Chanarang R, Surat Wadee J, Weerapol J (1988) Effective plant crude extract on tick (B. Microphilus)-Larvicidal action. Kasetstart Jl Nat Sci Suppl 22:37–41

    Google Scholar 

  • Nari A (1995) Stratigies for control of one host ticks and relationship with tick borne diseases in South Africa. Vet Parasitol 57:153–165

    Article  PubMed  CAS  Google Scholar 

  • Nongluck S, Wandee G, Aim-On S, Lekcharoen K, Pongkrit T (2006) Toxicity testing of organic solvent extracts from Annona squamosa: effects on rabbit eyes and ear skin. Phytother Res 8:365–368

    Google Scholar 

  • Shankaram AVB, Murthy MM, Akkewar DM, Subramanyam M, Rao AN (2006) United States patent no. 6,991,818.

  • Snedecor GW, Cochran WG (1968) Statistical methods. Oxford and IBH, Calcutta, India, pp 1–593

    Google Scholar 

  • Srivastava R, Ghosh S, Mondal DB, Azhahianambi P, Singhal PSD, Pandey NN, Swarup D (2008) Efficacy of Azadirachta indica extracts against Boophilus microplus. Parasitol Res 104:149–153

    Article  PubMed  Google Scholar 

  • WHO (1993) Vector resistance to pesticide. Fifteenth Report of the WHO Expert Committee on Vector Biology and Control. Technical Repot Service, pp 818, 58, WHO, Geneva.

Download references

Acknowledgement

Sincere thanks are due to Indian Council of Agricultural Research for providing financial support for conducting the experiment. The publication of the work is facilitated through National Agricultural Innovation Project No. NAIP/Comp-4/C2066/2007-08.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to S. Ghosh.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Magadum, S., Mondal, D.B. & Ghosh, S. Comparative efficacy of Annona squamosa and Azadirachta indica extracts against Boophilus microplus Izatnagar isolate. Parasitol Res 105, 1085–1091 (2009). https://doi.org/10.1007/s00436-009-1529-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-009-1529-3

Keywords

Navigation