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Polymerase chain reaction in the diagnosis of congenital toxoplasmosis: more than two decades of development and evaluation

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Abstract

Toxoplasmosis, a protozoan disease caused by the coccidian parasite Toxoplasma gondii, is one of the most prevalent parasitic diseases of humans. Although most infections are subclinical and asymptomatic, it has a great importance with respect to immunocompromized hosts and congenitally infected newborns. The diagnosis of T. gondii infection has gained in great interest over the past decades with a wide variety of techniques being investigated from animal inoculation to advanced molecular techniques. This paper reviews and highlights the development and evaluation of polymerase chain reaction in the diagnosis of congenital toxoplasmosis in human population to present the redundant research on it in a chronologically simple approach.

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References

  • Antsaklis A, Daskalakis G, Papantoniou N, Mentis A, Michalas S (2002) Prenatal diagnosis of congenital toxoplasmosis. Prenat Diagn 22:1107–1111

    Article  PubMed  Google Scholar 

  • Araujo FG, Williams DM, Grumet FC, Remington JS (1976) Strain-dependent differences in murine susceptibility to Toxoplasma. Infect Immun 13:1528–1530

    CAS  PubMed  Google Scholar 

  • Assmar M, Yassaei F, Terhovanesian A, Esmaeili AR, Hassan N, Farzanehnezhaad Z et al (2004) Prenatal diagnosis of congenital toxoplasmosis: validity of PCR using amniotic fluid against indirect fluorescent antibody assay in mothers. Iran J Publ Health 33:1–4

    Google Scholar 

  • Bastien P (2002) Molecular diagnosis of toxoplasmosis. Trans R Soc Trop Med Hyg 96:S205–S215

    Article  PubMed  Google Scholar 

  • Bessières MH, Berrebi A, Cassaing S, Fillaux J, Cambus JP, Berry A et al (2009) Diagnosis of congenital toxoplasmosis: prenatal and neonatal evaluation of methods used in Toulouse University Hospital and incidence of congenital toxoplasmosis. Mem Inst Oswaldo Cruz 104:389–392

    Article  PubMed  Google Scholar 

  • Burg JL, Perelman D, Kasper LH, Ware PL, Boothroyd JC (1988) Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii. J Immunol 141:3584–3591

    CAS  PubMed  Google Scholar 

  • Burg JL, Grover CM, Pouletty P, Boothroyd JC (1989) Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction. J Clin Microbiol 27:1787–1792

    CAS  PubMed  Google Scholar 

  • Cazenave J, Bessieres MH, Sourrue D, Cheyrou A, Begueret J (1990) Detection de Toxoplasmes par amplification d'ADN. Un exemple d'application des techniques de biologie mole´culaire au diagnostic parasitaire. Rev Fr Lab 209:118–126

    Google Scholar 

  • Cazenave J, Forestier F, Bessieres MH, Broussin B, Begueret J (1992) Contribution of a new PCR assay to the prenatal diagnosis of congenital toxoplasmosis. Prenat Diagn 12:119–127

    Article  CAS  PubMed  Google Scholar 

  • Costa JM, Dardé ML, Assouline B, Vidaud M, Bretagne S (1997) Microsatellite in the beta-tubulin gene of Toxoplasma gondii as a new genetic marker for use in direct screening of amniotic fluids. J Clin Microbiol 35:2542–2545

    CAS  PubMed  Google Scholar 

  • Costa JM, Ernault P, Gautier E, Bretagne S (2001) Prenatal diagnosis of congenital toxoplasmosis by duplex real-time PCR using fluorescence resonance energy transfer hybridization probes. Prenat Diagn 21:85–88

    Article  CAS  PubMed  Google Scholar 

  • Cristina N, Derouin F, Pelloux H, Pierce R, Cesbron-Delauwn MF, Ambroise-Thomas P (1992) Detection of Toxoplasma gondii by “Polymerase Chain Reaction” (PCR) technique in AIDS infected patients using the repetitive sequence TGR1E. Pathol Biol (Paris) 40:52–55

    CAS  Google Scholar 

  • Daffos F, Capella-Pavlovsky M, Forestier F (1985) Fetal blood sampling during pregnancy with use of a needle guided by ultrasound: a study of 606 consecutive cases. Am J Obstet Gynecol 153:655–660

    CAS  PubMed  Google Scholar 

  • Daffos F, Forestier F, Capella-Pavlovsky M, Thulliez P, Aufrant C, Valenti D et al (1988) Prenatal management of 746 pregnancies at risk for congenital toxoplasmosis. N Engl J Med 318:271–275

    Article  CAS  PubMed  Google Scholar 

  • Dalgıç N (2008) Congenital Toxoplasma gondii infection. Marmara Med J 21:89–101

    Google Scholar 

  • Derouin F, Thulliez P, Candolfi E, Daffos F, Forestier F (1988) Early prenatal diagnosis of congenital toxoplasmosis using amniotic fluid samples and tissue culture. Eur J Clin Microbiol 7:423–425

    Article  CAS  Google Scholar 

  • Desmonts G, Daffos F, Forestier F, Capella-Pavlovsky M, Thulliez P, Chartier M (1985) Prenatal diagnosis of congenital toxoplasmosis. Lancet 1:500–504

    Article  CAS  PubMed  Google Scholar 

  • Dupouy-Camet J, de Souza SL, Maslo C, Paugam A, Saimot AG, Benarous R et al (1993) Detection of Toxoplasma gondii in venous blood from AIDS patients by polymerase chain reaction. J Clin Microbiol 31:1866–1869

    CAS  PubMed  Google Scholar 

  • Eida OM, Eida MM, Ahmed AB (2009) Evaluation of polymerase chain reaction on amniotic fluid for diagnosis of congenital toxoplasmosis. J Egypt Soc Parasitol 39:541–550

    PubMed  Google Scholar 

  • Filisetti D, Gorcii M, Pernot-Marino E, Villard O, Candolfi E (2003) Diagnosis of congenital toxoplasmosis: comparison of targets for detection of Toxoplasma gondii by PCR. J Clin Microbiol 41:4826–4828

    Article  CAS  PubMed  Google Scholar 

  • Foulon W, Pinon JM, Stray-Pedersen B, Pollak A, Lappalainen M, Decoster A et al (1999) Prenatal diagnosis of congenital toxoplasmosis: a multicenter evaluation of different diagnostic parameters. Am J Obstet Gynecol 181:843–847

    Article  CAS  PubMed  Google Scholar 

  • Fricker-Hidalgo H, Pelloux H, Racinet C, Grefenstette I, Bost-Bru C, Goullier-Fleuret A et al (1998) Detection of Toxoplasma gondii in 94 placentae from infected women by polymerase chain reaction, in vivo, and in vitro cultures. Placenta 19:545–549

    Article  CAS  PubMed  Google Scholar 

  • Fuentes I, Rodriguez M, Domingo CJ, del Castillo F, Juncosa T, Alvar J (1996) Urine sample used for congenital toxoplasmosis diagnosis by PCR. J Clin Microbiol 34:2368–2371

    CAS  PubMed  Google Scholar 

  • Gola J, Czuba B, Mazurek U, Sławska H, Kamiński K, Wilczok T (2000) Usefulness of quantitative assessment of Toxoplasma gondii genome using PCR-TaqMan in amniotic fluid, maternal and neonatal blood in selected complications in pregnancy. Ginekol Pol 71:954–958

    CAS  PubMed  Google Scholar 

  • Gratzl R, Hayde M, Kohlhauser C, Hermon M, Burda G, Strobl W et al (1998) Follow-up of infants with congenital toxoplasmosis detected by polymerase chain reaction analysis of amniotic fluid. Eur J Clin Microbiol Infect Dis 17:853–858

    Article  CAS  PubMed  Google Scholar 

  • Gross U, Müller J, Roos T, Schrod L, Heesemann J (1992) Possible reasons for failure of conventional tests for diagnosis of fatal congenital toxoplasmosis: report of a case diagnosed by PCR and immunoblot. Infection 20:149–152

    Article  CAS  PubMed  Google Scholar 

  • Grover CM, Thulliez P, Remington JS, Boothroyd JC (1990) Rapid prenatal diagnosis of congenital Toxoplasma infection by using polymerase chain reaction and amniotic fluid. J Clin Microbiol 28:2297–2301

    CAS  PubMed  Google Scholar 

  • Guay JM, Dubois D, Morency MJ, Gagnon S, Mercier J, Levesque RC (1993) Detection of the pathogenic parasite Toxoplasma gondii by specific amplification of ribosomal sequences using comultiplex polymerase chain reaction. J Clin Microbiol 31:203–207

    CAS  PubMed  Google Scholar 

  • Guy EC, Pelloux H, Lappalainen M, Aspöck H, Hassl A, Melby KK et al (1996) Interlaboratory comparison of polymerase chain reaction for the detection of Toxoplasma gondii DNA added to samples of amniotic fluid. Eur J Clin Microbiol Infect Dis 15:836–839

    Article  CAS  PubMed  Google Scholar 

  • Hohlfeld P, Daffos F, Thulliez P, Aufrant C, Couvreur J, MacAleese J et al (1989) Fetal toxoplasmosis: outcome of pregnancy and infant follow-up after in utero treatment. J Pediatr 115:765–769

    Article  CAS  PubMed  Google Scholar 

  • Hohlfeld P, Daffos F, Costa JM, Thulliez P, Forestier F, Vidaud M (1994) Prenatal diagnosis of congenital toxoplasmosis with a polymerase-chain-reaction test on amniotic fluid. N Engl J Med 331:695–699

    Article  CAS  PubMed  Google Scholar 

  • Homan WL, Vercammen M, De Braekeleer J, Verschueren H (2000) Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR. Int J Parasitol 30:69–75

    Article  CAS  PubMed  Google Scholar 

  • Jenum PA, Holberg-Petersen M, Melby KK, Stray-Pedersen B (1998) Diagnosis of congenital Toxoplasma gondii infection by polymerase chain reaction (PCR) on amniotic fluid samples. The Norwegian experience. APMIS 106:680–686

    Article  CAS  PubMed  Google Scholar 

  • Joss AW, Ho-Yen DO (1997) The effect of sample storage on polymerase chain reaction-based detection of Toxoplasma gondii in amniotic fluids. J Med Microbiol 46:92–96

    Article  CAS  PubMed  Google Scholar 

  • Kasper DC, Sadeghi K, Prusa AR, Reischer GH, Kratochwill K, Förster-Waldl E et al (2009) Quantitative real-time polymerase chain reaction for the accurate detection of Toxoplasma gondii in amniotic fluid. Diagn Microbiol Infect Dis 63:10–15

    Article  CAS  PubMed  Google Scholar 

  • Knerer B, Hayde M, Gratzl R, Strobl W, Pollak A (1995) Direct detection of Toxoplasma gondii with polymerase chain reaction in diagnosis of fetal Toxoplasma infection. Wien Klin Wochenschr 107:137–140

    CAS  PubMed  Google Scholar 

  • Ma YY, Mu L, Wang LY, Jiang S (2003) Study on prenatal diagnosis using fluorescence quantitative polymerase chain reaction for CT. Zhonghua Fu Chan Ke Za Zhi 38:8–10

    PubMed  Google Scholar 

  • Menotti J, Garin YJ, Thulliez P, Sérugue MC, Stanislawiak J, Ribaud P et al (2010) Evaluation of a new 5'-nuclease real-time PCR assay targeting the Toxoplasma gondii AF146527 genomic repeat. Clin Microbiol Infect 16:363–368

    Article  CAS  PubMed  Google Scholar 

  • Nagy B, Bán Z, Beke A, Nagy GR, Lázár L, Papp C et al (2006) Detection of Toxoplasma gondii from amniotic fluid, a comparison of four different molecular biological methods. Clin Chim Acta 368:131–137

    Article  CAS  PubMed  Google Scholar 

  • Nowakowska D, Respondek-Liberska M, Golab E, Stray-Pedersen B, Szaflik K, Dzbenski TH et al (2005) Too late prenatal diagnosis of fetal toxoplasmosis: a case report. Fetal Diagn Ther 20:190–193

    Article  PubMed  Google Scholar 

  • Okay TS, Yamamoto L, Oliveira LC, Manuli ER, Andrade Junior HF et al (2009) Significant performance variation among PCR systems in diagnosing congenital toxoplasmosis in São Paulo, Brazil: analysis of 467 amniotic fluid samples. Clin Sao Paulo 64:171–176

    Google Scholar 

  • Ordinaire I, Simon A, Fréalle E, Soula F, Valat AS, Rouland V et al (2005) Real-time quantitative PCR for toxoplasmosis diagnosis. Ann Biol Clin (Paris) 63:67–73

    CAS  Google Scholar 

  • Pelloux H, Weiss J, Simon J, Muet F, Fricker-Hidalgo H, Goullier-Fleuret A et al (1996) A new set of primers for the detection of Toxoplasma gondii in amniotic fluid using polymerase chain reaction. FEMS Microbiol Lett 138:11–15

    Article  CAS  PubMed  Google Scholar 

  • Pelloux H, Guy E, Angelici MC, Aspöck H, Bessières MH, Blatz R et al (1998) A second European collaborative study on polymerase chain reaction for Toxoplasma gondii, involving 15 teams. FEMS Microbiol Lett 165:231–237

    Article  CAS  PubMed  Google Scholar 

  • Peyron F, Eudes N, de Monbrison F, Wallon M, Picot S (2004) Fitness of Toxoplasma gondii is not related to DHFR single-nucleotide polymorphism during congenital toxoplasmosis. Int J Parasitol 34:1169–1175

    Article  CAS  PubMed  Google Scholar 

  • Remington JS, Desmonts G (1990) Toxoplasmosis. In: Remington JS, Klein JO (eds) Infectious diseases of the fetus and newborn infant. W.B. Saunders, Philadelphia, pp 89–195

    Google Scholar 

  • Robert-Gangneux F, Dupretz P, Yvenou C, Quinio D, Poulain P, Guiguen C et al (2010) Clinical relevance of placenta examination for the diagnosis of congenital toxoplasmosis. Pediatr Infect Dis J 29:33–38

    Article  PubMed  Google Scholar 

  • Romand S, Wallon M, Franck J, Thulliez P, Peyron F, Dumon H (2001) Prenatal diagnosis using polymerase chain reaction on amniotic fluid for congenital toxoplasmosis. Obstet Gynecol 97:296–300

    Article  CAS  PubMed  Google Scholar 

  • Romand S, Chosson M, Franck J, Wallon M, Kieffer F, Kaiser K et al (2004) Usefulness of quantitative polymerase chain reaction in amniotic fluid as early prognostic marker of fetal infection with Toxoplasma gondii. Am J Obstet Gynecol 190:797–802

    Article  CAS  PubMed  Google Scholar 

  • Savva D, Morris JC, Johnson JD, Holliman RE (1990) Polymerase chain reaction for detection of Toxoplasma gondii. J Med Microbiol 32:25–31

    Article  CAS  PubMed  Google Scholar 

  • Suzuki Y, Takemoto O, Arai H, Goto M, Yamada J, Morimoto K et al (1998) A case of congenital toxoplasmosis confirmed by detection of Toxoplasma gondii in placenta. No To Hattatsu 30:411–416

    CAS  PubMed  Google Scholar 

  • Swisher CN, Boyer K, McLeod R (1994) Congenital toxoplasmosis. The Toxoplasmosis Study Group. Sem Pediatr Neurol 1:4–25

    CAS  Google Scholar 

  • Thalib L, Gras L, Romand S, Prusa A, Bessieres MH, Petersen E et al (2005) Prediction of congenital toxoplasmosis by polymerase chain reaction analysis of amniotic fluid. BJOG 112:567–574

    Article  CAS  PubMed  Google Scholar 

  • Tóth T, Sziller I, Papp Z (1998) PCR detection of Toxoplasma gondii in human fetal tissues. In: Meltzer SJ (ed) PCR in bioanalysis: Methods in molecular biology Vol. 92. Humana Press, New Jeresy, pp 195–202

    Chapter  Google Scholar 

  • Wahab T, Edvinsson B, Palm D, Lindh J (2010) Comparison of the AF146527 and B1 repeated elements, two real-time PCR targets used for detection of Toxoplasma gondii. J Clin Microbiol 48:591–592

    Article  CAS  PubMed  Google Scholar 

  • Wallon M, Franck J, Thulliez P, Huissoud C, Peyron F, Garcia-Meric P, Kieffer F et al (2010) Accuracy of real-time polymerase chain reaction for Toxoplasma gondii in amniotic fluid. Obstet Gynecol 115:727–733

    Article  PubMed  Google Scholar 

  • Wang X, Zhang GC, Han M (2001) Detection of TORCH genom in the cardiac tissue of congenital heart disease. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 15:176–178

    CAS  PubMed  Google Scholar 

  • Weiss LM, Udem SA, Salgo M, Tanowitz HB, Wittner M (1991) Sensitive and specific detection of Toxoplasma DNA in an experimental murine model: use of Toxoplasma gondii-specific cDNA and the polymerase chain reaction. J Infect Dis 163:180–186

    CAS  PubMed  Google Scholar 

  • Yamakawa R, Yamashita Y, Yano A, Morita J, Kato H (1996) Congenital toxoplasmosis complicated by central diabetes insipidus in an infant with Down syndrome. Brain Dev 18:75–77

    Article  CAS  PubMed  Google Scholar 

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  1. Faculty of Medicine and Health Sciences, Department of Medical Parasitology, Sana’a University, Sana’a, Yemen

    Rashad Abdul-Ghani

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  1. Rashad Abdul-Ghani
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Correspondence to Rashad Abdul-Ghani.

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Abdul-Ghani, R. Polymerase chain reaction in the diagnosis of congenital toxoplasmosis: more than two decades of development and evaluation. Parasitol Res 108, 505–512 (2011). https://doi.org/10.1007/s00436-010-2245-8

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