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Prominin-1/CD133, a neural and hematopoietic stem cell marker, is expressed in adult human differentiated cells and certain types of kidney cancer

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Abstract

Human prominin-1/CD133 has been reported to be expressed in neural and hematopoietic stem/progenitor cells and in embryonic, but not adult, epithelia. This lack of detection of human prominin-1, as defined by its glycosylation-dependent AC133 epitope, is surprising given the expression of the murine ortholog in adult epithelia. Here, we demonstrate, by using a novel prominin-1 antiserum (αhE2), that the decrease of AC133 immunoreactivity observed during differentiation of the colonic adenocarcinoma-derived Caco-2 cells is not paralleled by a down-regulation of prominin-1. We have also shown that αhE2 immunoreactivity, but not AC133 immunoreactivity, is present in several adult human tissues, such as kidney proximal tubules and the parietal layer of Bowman’s capsule of juxtamedullary nephrons, and in lactiferous ducts of the mammary gland. These observations suggest that only the AC133 epitope is down-regulated upon cell differentiation. Furthermore, αhE2 immunoreactivity has been detected in several kidney carcinomas derived from proximal tubules, independent of their grading. Interestingly, in one particular case, the AC133 epitope, which is restricted to stem cells in normal adult tissue, was up-regulated in the vicinity of the tumor. Our data thus show that (1) in adults, the expression of human prominin-1 is not limited to stem and progenitor cells, and (2) the epitopes of prominin-1 might be useful for investigating solid cancers.

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Acknowledgements

We thank D. Buck for the human prominin-1 cDNA, G. Baretton for human tissue samples, I. Peterson for her excellent sample preparation, I. Nüβlein for her valuable assistance with FACS, and S. Langer for valuable technical assistance with some immunohistochemistry experiments.

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Authors and Affiliations

  1. Medical Clinic and Polyclinic I, University Carl Gustav Carus, Fetscherstrasse 74, 01307, Dresden, Germany

    Mareike Florek, Daniel Freund, Gerhard Ehninger & Denis Corbeil

  2. Department of Pathology, University Carl Gustav Carus, Fetscherstrasse 74, 01307, Dresden, Germany

    Michael Haase

  3. Max-Planck-Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307, Dresden, Germany

    Anne-Marie Marzesco, Wieland B. Huttner & Denis Corbeil

Authors
  1. Mareike Florek
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  2. Michael Haase
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  3. Anne-Marie Marzesco
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  4. Daniel Freund
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  5. Gerhard Ehninger
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  6. Wieland B. Huttner
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  7. Denis Corbeil
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Corresponding author

Correspondence to Denis Corbeil.

Additional information

This study was supported by grants from the Deutsche Forschungsgemeinschaft (SPP 1109, CO 298/2-1 to D.C., Hu 275/7-1 to W.B.H.; SPP 1111, Hu 275/8-2 to W.B.H.) and the Fonds der Chemischen Industrie (to W.B.H.)

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Florek, M., Haase, M., Marzesco, AM. et al. Prominin-1/CD133, a neural and hematopoietic stem cell marker, is expressed in adult human differentiated cells and certain types of kidney cancer. Cell Tissue Res 319, 15–26 (2005). https://doi.org/10.1007/s00441-004-1018-z

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  • DOI: https://doi.org/10.1007/s00441-004-1018-z

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