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Stromal cells cultured from omentum express pluripotent markers, produce high amounts of VEGF, and engraft to injured sites

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Abstract

When rat omentum becomes activated by intraperitoneal injection of inert polydextran particles, these particles are rapidly surrounded by cells that express markers of adult stem cells (SDF–1α, CXCR4, WT–1) and of embryonic pluripotent cells (Oct–4, Nanog, SSEA–1). We have cultured such cells, because they may offer a convenient source of adult stem cells, and have found that they retain stem cell markers and produce high levels of vascular endothelial growth factor for up to ten passages. After systemic or local injection of these cultured cells into rats with acute injury of various organs, the cells specifically engraft at the injured sites. Thus, our experiments show that omental stromal cells can be cultured from activated omentum, and that these cells exhibit stem cell properties enabling them to be used for repair and possibly for the regeneration of damaged tissues.

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Acknowledgements

The authors thank Ms. Linda Wanna for culture work and Dr. Lev Rappoport for help with histological processing and immunostaining of tissues.

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Authors and Affiliations

  1. The Division of Nephrology, John Stroger Jr. Hospital of Cook County, Chicago, IL, USA

    Ashok K. Singh, Krishnamurthy P. Gudehithlu, Jose A. L. Arruda & George Dunea

  2. Section of Nephrology, University of Illinois at Chicago and the Chicago VAMC, Chicago, IL, USA

    Jose A. L. Arruda

  3. Hektoen Institute of Medicine, Chicago, IL, USA

    Ashok K. Singh, Jilpa Patel, Perianna Sethupathi & George Dunea

  4. Section of Nephrology, Loyola-Hines Medical Center, Maywood, IL, USA

    Natalia O. Litbarg

  5. Stroger Hospital of Cook County, 637 South Wood St (Durand Bldg, 2nd Floor), Chicago, 60612, IL, USA

    Ashok K. Singh

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  1. Ashok K. Singh
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  2. Jilpa Patel
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  3. Natalia O. Litbarg
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Correspondence to Ashok K. Singh.

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Singh, A.K., Patel, J., Litbarg, N.O. et al. Stromal cells cultured from omentum express pluripotent markers, produce high amounts of VEGF, and engraft to injured sites. Cell Tissue Res 332, 81–88 (2008). https://doi.org/10.1007/s00441-007-0560-x

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  • DOI: https://doi.org/10.1007/s00441-007-0560-x

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