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Aquaporin-1 in blood vessels of rat circumventricular organs

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Abstract

Although the water channel protein aquaporin-1 (AQP1) is widely observed outside the rat brain in continuous, but not fenestrated, vascular endothelia, it has not previously been observed in any endothelia within the normal rat brain and only to a limited extent in the human brain. In this immunohistochemical study of rat brain, AQP1 has also been found in microvessel endothelia, probably of the fenestrated type, in all circumventricular organs (except the subcommissural organ and the vascular organ of the lamina terminalis): in the median eminence, pineal, subfornical organ, area postrema and choroid plexus. The majority of microvessels in the median eminence, pineal and choroid plexus, known to be exclusively fenestrated, are shown to be AQP1-immunoreactive. In the subfornical organ and area postrema in which many, but not all, microvessels are fenestrated, not all microvessels are AQP1-immunoreactive. In the AQP1-immunoreactive microvessels, the AQP1 probably facilitates water movement between blood and interstitium as one component of the normal fluxes that occur in these specialised sensory and secretory areas. AQP1-immunoreactive endothelia have also been seen in a small population of blood vessels in the cerebral parenchyma outside the circumventricular organs, similar to other observations in human brain. The proposed development of AQP1 modulators to treat various brain pathologies in which AQP1 plays a deleterious role will necessitate further work to determine the effect of such modulators on the normal function of the circumventricular organs.

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Acknowledgements

Renata Billing provided skilled technical assistance. Dr. I.J. Llewellyn-Smith kindly provided a critical review of the manuscript.

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Authors and Affiliations

  1. Department of Anatomy and Histology, Centre for Neuroscience, School of Medicine, Flinders University, Bedford Park, SA, 5042, Australia

    Alan J. Wilson, Colin J. Carati, Bren J. Gannon & Rainer Haberberger

  2. Department of Human Physiology, Centre for Neuroscience, School of Medicine, Flinders University, Bedford Park, SA, Australia

    Tim K. Chataway

Authors
  1. Alan J. Wilson
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  2. Colin J. Carati
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  3. Bren J. Gannon
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  4. Rainer Haberberger
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  5. Tim K. Chataway
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Corresponding author

Correspondence to Alan J. Wilson.

Additional information

Bren J. Gannon died 13/10/09.

This work was supported by a Flinders Institute for Health and Medical Research Competitive Research Grant.

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Wilson, A.J., Carati, C.J., Gannon, B.J. et al. Aquaporin-1 in blood vessels of rat circumventricular organs. Cell Tissue Res 340, 159–168 (2010). https://doi.org/10.1007/s00441-010-0927-2

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  • DOI: https://doi.org/10.1007/s00441-010-0927-2

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