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Proteasomal degradation of ubiquitinated proteins in oocyte meiosis and fertilization in mammals

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Abstract

Gametogenesis and fertilization are the key events in sexual reproduction. In the female, meiosis results in a large oocyte that is competent for fertilization and fundamental for the success of early embryonic development. Progression through meiosis is monitored by fine regulatory mechanisms. In this review, we focus on one of the most well-known regulatory elements, the E3 ligase APC/C, which mediates proteolytic degradation of a number of important substrates via the ubiquitin proteasome pathway (UPP). The UPP also indirectly regulates protein synthesis by affecting proteins involved in RNA metabolism, a process that is paramount for the transcriptionally silent oocyte. During the past few years, more evidence has accumulated to suggest that the UPP has an important role in zona pellucida penetration and gamete fusion in mammals. This review focuses on the function of the UPP in regulating oocyte meiotic maturation in mammals, with special attention to its role in chromosome segregation and polar body extrusion, its role in the acquisition of meiotic/developmental competence and recent advances in our understanding of the UPP role in fertilization.

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Acknowledgment

We would like to thank Dr. Peter Sutovsky for helpful comments on the manuscript, Dr. Matthew S. Cook for careful reading of the manuscript and Ms. Kathy Craighead for manuscript editing. This work was supported by P502/10/0944 for M.K. and P.K. was supported by grant 204/09/H084, both from the Czech Science Foundation.

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  1. Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Rumburska 89, 277 21, Libechov, Czech Republic

    Pavla Karabinova & Michal Kubelka

  2. Department of Obstetrics, Gynecology, and Reproductive Sciences, Center for Reproductive Sciences, University of California, San Francisco, San Francisco, CA, 94143, USA

    Andrej Susor

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  1. Pavla Karabinova
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  2. Michal Kubelka
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Correspondence to Andrej Susor.

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Karabinova, P., Kubelka, M. & Susor, A. Proteasomal degradation of ubiquitinated proteins in oocyte meiosis and fertilization in mammals. Cell Tissue Res 346, 1–9 (2011). https://doi.org/10.1007/s00441-011-1235-1

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