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Lesson from the GFP/CCl4 model — Translational Research Project: the development of cell therapy using autologous bone marrow cells in patients with liver cirrhosis

  • Published:
Journal of Hepato-Biliary-Pancreatic Surgery

Abstract

The plasticity of bone marrow has been confirmed by the analysis of autopsy findings in female recipients of bone marrow cells transplanted from male donors. To establish new clinical cell therapies using autologous bone marrow cells for patients with liver failure, we developed a new in vivo model, the “green fluorescent protein (GFP)/carbon tetrachloride (CCl4) model”. Using the GFP/CCl4 model, we found that transplanted Liv8-negative cells efficiently repopulated into cirrhotic liver tissue and trans-differentiated into albumin-producing hepatocytes under conditions of persistent liver damage induced by CCl4. Moreover, one marrow cell transplantation into liver cirrhosis mice improved their liver function, ameliorated liver fibrosis, and improved their survival rate. Results from the GFP/CCl4 model showed that cell therapy using autologous bone marrow cells has the potential to become an effective treatment for patients with liver failure. Here we describe the findings from the GFP/CCl4 model and the scope of the translational research project.

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Authors and Affiliations

  1. Department of Molecular Science and Applied Medicine (Gastroenterology and Hepatology), Yamaguchi University School of Medicine, 1-1-1 Minami Kogushi, Ube, Yamaguchi, 755-8505, Japan

    Shuji Terai, Isao Sakaida & Kiwamu Okita

  2. Department of Developmental and Regenerative Biology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan

    Hiroshi Nishina

Authors
  1. Shuji Terai
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  2. Isao Sakaida
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  3. Hiroshi Nishina
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  4. Kiwamu Okita
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Terai, S., Sakaida, I., Nishina, H. et al. Lesson from the GFP/CCl4 model — Translational Research Project: the development of cell therapy using autologous bone marrow cells in patients with liver cirrhosis. J Hepatobiliary Pancreat Surg 12, 203–207 (2005). https://doi.org/10.1007/s00534-005-0977-0

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  • DOI: https://doi.org/10.1007/s00534-005-0977-0

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