Abstract
Hydatidosis in humans and animals is a substantial cause of deaths in many parts of the world. Surgery and the chemotherapeutic agents are the main treatments for hydatid disease; however, most of them are accompanied by adverse side effects. In this study, the scolicidal effect of essential oils from Zataria multiflora and Ferula assafoetida against Echinococcus granulosus protoscolices was investigated. Essential oils from Z. multiflora and F. assafoetida were prepared by hydrodistillation and analyzed by gas chromatography–mass spectrometry. Carvacrol (29.2 %), thymol (25.4 %), p-cymene (11.2 %), linalool (9.6 %), and γ-terpinene (8 %) were detected as the main components of the Z. multiflora, while the main components of F. assafoetida were E-1-propenyl-sec-butyl disulfide (62.7 %), β-ocimene (21.7 %) and β-pinene (5 %). Scolicidal activities of the essential oils against protoscolices for Z. multiflora and F. assafoetida were obtained at concentrations more than 17 and 60 μg/mL, respectively. Accordingly, it was concluded that both essential oils can selectively reduce protoscolices viability, and Z. multiflora, which contains phenolic monoterpenes, is more cytotoxic rather than F. assafoetida which contains disulfide compounds. Therefore, Z. multiflora and F. assafoetida could be recommended as a treatment against hydatid cyst.
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References
Adams RP (2007) Identification of essential oil components by gas chromatography/mass spectrometry, 4th edn. Allured Publishing Corporation, IL
Adas G, Arikan S, Kemik O, Oner A, Sahip N, Karatepe O (2009) Use of albendazole sulfoxide, albendazole sulfone, and combined solutions as scolicidal agents on hydatid cysts (in vitro study). World J Gastroenterol 15:112–116
Albi A, Baudin F, Matmar M, Archambeau D, Ozier Y (2002) Severe hypernatremia after hypertonic saline irrigation of hydatid cysts. Anesth Analg 95:1806–1808
Amirghofran Z, Hashemzadeh R, Javidnia K, Golmoghaddam H, Esmaeilbeig A (2011) In vitro immunomodulatory effects of extracts from three plants of the Labiatae family and isolation of the active compounds. J Immunotoxicol 8:265–273
Anthony JP, Fyfe L, Smith H (2005) Plant active components. A resource for antiparasitic agents? Trends Parasitol 21:462–468
Arunasree KM (2010) Anti-proliferative effects of carvacrol on a human metastatic breast cancer cell line, MDA-MB 231. Phytomedicine 17:581–588
Bagheri SM, Sahebkar A, Gohari AR, Saeidnia S, Malmir M, Iranshahi M (2010) Evaluation of cytotoxicity and anticonvulsant activity of some Iranian medicinal Ferula species. Pharm Biol 48:242–246
Bakkali F, Averbeck S, Averbeck D, Idaomar M (2008) Biological effects of essential oils. Food Chem Toxicol 46:447–475
Baser KHC, Demirci F (2007) Chemistry of essential oils. In: Berger RG (ed) Flavours and fragrances: chemistry, bioprospecting and sustainability. Springer, Heidelberg, pp 43–86
Besim H, Karayalcin K, Hamamci O, Gungor C, Korkmaz A (1998) Scolicidal agents in hydatid cyst surgery. HPB Surg 10:347–351
Caglar R, Yuzbasioglu MF, Bulbuloglu E, Gul M, Ezberci F, Kale IT (2008) In vitro effectiveness of different chemical agents on scolices of hydatid cyst. J Invest Surg 21:71–75
Chang HT, Hsu SS, Chou CT, Cheng JS, Wang JL, Lin KL, Fang YC, Chen WC, Chien JM, Lu T, Pan CC, Cheng HH, Huang JK, Kuo CC, Chai KL, Jan CR (2011) Effect of thymol on calcium homeostasis and viability in MG63 human osteosarcoma cells. Pharmacology 88:201–212
Craig PS, McManus DP, Lightowlers MW, Chabalgoity JA, Garcia HH, Gavidia CM, Golman RH, Gonzalez AE, Lorca M, Naquira C (2007) Prevention and control of cystic echinococcosis. Lancet Infect Dis 7:385–394
Dall Acqua S, Linardi MA, Maggi F, Nicoletti M, Petitto V, Innocenti G, Basso G, Viola G (2011) Natural daucane sesquiterpenes with antiproliferative and proapoptotic activity against human tumor cells176. Bioorg Med Chem 19:5876–5885
Deb DD, Parimala G, Saravana DS, Chakraborty T (2011) Effect of thymol on peripheral blood mononuclear cell PBMC and acute promyelotic cancer cell line HL-60. Chem Biol Interact 193:97–106
Diaz C, Quesada S, Brenes O, Aguilar G, Ciccio JF (2008) Chemical composition of Schinus molle essential oil and its cytotoxic activity on tumor cell lines. Nat Prod Res 22:1521–1534
Dorman HJD, Deans SG (2000) Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J Appl Microbiol 88:308–316
Erzurumlu K, Ozdemir M, Mihmanli M, Cevikbay U (1998) Effect of albendazole sulfoxide solution on the scolices and the hepatobiliary system. Eur Surg Res 30:433–438
Griffin SG, Wyllie SG, Markham JL, Leach D (1999) The role of structure and molecular properties of terpenoids in determining their antimicrobial activity. Flavour Fragr 14:322–332
Hanafi-Bojd MY, Iranshahi M, Mosaffa F, Tehrani SO, Kalalinia F, Behravan J (2011) Farnesiferol A from Ferula persica and galbanic acid from Ferula szowitsiana inhibit P-glycoprotein-mediated rhodamine efflux in breast cancer cell lines. Planta Med 77:1590–1593
Harikumar KB, Aggarwal BB (2008) Resveratrol: a multitargeted agent for age-associated chronic diseases. Cell Cycle 7:1020–1035
Hsu SS, Lin KL, Chou CT, Chiang AJ, Liang WZ, Chang HT, Tsai JY, Liao WC, Huang FD, Huang JK, Chen IS, Liu SI, Kuo CC, Jan CR (2011) Effect of thymol on calcium homeostasis and viability in human glioblastoma cells. Eur J Pharmacol 670:85–91
Hui H, Tang G, Go VLW (2009) Hyperglycemic herbs and their actions mechanisms. Chin Med 4:11–22
Iranshahy M, Iranshahi M (2011) Traditional uses, phytochemistry and pharmacology of asafoetida (Ferula assafoetida oleo-gum-resin)—a review. J Ethnopharmacol 134:1–10
Kahriman G, Ozcan N, Donmez H (2011) Hydatid cysts of the liver in children: percutaneous treatment with ultrasound follow-up. Pediatr Radiol 41:890–894
Kim KH, Lee HJ, Jeong SJ, Lee HJ, Lee EO, Kim HS, Zhang Y, Ryu SY, Lee MH, Lü J, Kim SH (2011) Galbanic acid isolated from F. assafoetida exerts in vivo anti-tumor activity in association with anti-angiogenesis and anti-proliferation. Pharm Res 28:597–609
Kuete V, Wiench B, Hegazy ME, Mohamed TA, Fankam AG, Shahat AA, Efferth T (2011) Antibacterial activity and cytotoxicity of selected Egyptian medicinal plants. Planta Med 78:193–199
Kumar A, Malik F, Bhushan S, Sethi VK, Shahi AK, Kaur J, Taneja SC, Qazi GN, Singh J (2008) An essential oil and its major constituent isointermedeol induce apoptosis by increased expression of mitochondrial cytochrome c and apical death receptors in human leukemia HL-60 cells. Chem Biol Interact 171:332–347
Landa-Garcia JI, Alonso E, Gonzalez-Uriarte J, Rodriguez-Romano D (1997) Evaluation of scolicidal agents in an experimental hydatid disease model. Eur Surg Res 29:202–208
Mazzio EA, Soliman KF (2011) In vitro screening of tumoricidal properties of international medicinal herbs: part II. Phytother Res 24:1813–1824
McLafferty FW (2009) Wiley registry of mass spectral data, 9th edn. Wiley, Hoboken, p 662
Moazeni M, Alipour-Chaharmahali MR (2010) Echinococcus granulosus: in vitro effectiveness of warm water on protoscolices. Exp Parasitol 127:14–17
Moazeni M, Larki S (2010) In vitro effectiveness of acidic and alkaline solutions on scolices of hydatid cyst. Parasitol Res 106:853–856
Moazeni M, Nazer A (2010) In vitro effectiveness of garlic (Allium sativum) extract on scolices of hydatid cyst. World J Surg 34:2677–2681
Moazeni M, Roozitalab A (2010) High scolicidal effect of Zataria multiflora on protoscolices of hydatid cyst: an in vitro study. Comp Clin Pathol 19:1–6
Moazeni M, Saharkhiz MJ, Hoseini AA, Mootabi A (2012) In vitro scolicidal effect of Satureja khuzistanica (Jamzad) essential oil. Asian Pac J Trop Biomed 2:616–620
Mollazadeh S, Matin MM, Iranshahi M, Bahrami AR, Neshati V, Behnam-Rassouli F (2010) The enhancement of vincristine cytotoxicity by combination with feselol. J Asian Nat Prod Res 12:569–575
Nazari ZE, Iranshahi M (2011) Biologically active sesquiterpene coumarins from Ferula species. Phytother Res 25:315–323
Olaku O, White JD (2011) Herbal therapy use by cancer patients: a literature review on case reports. Eur J Cancer 47:508–514
Peres VF, Moura DJ, Sperotto AR, Damasceno FC, Caramao EB, Zini CA, Saffi J (2009) Chemical composition and cytotoxic, mutagenic and genotoxic activities of the essential oil from Piper gaudichaudianum Kunth leaves. Food Chem Toxicol 47:2389–2395
Pharmacopeia B (1988) HMSO, vol 2. Berman, London, pp 137–138
Rassouli FB, Matin MM, Iranshahi M, Bahrami AR (2011a) Investigating the cytotoxic and apoptosis inducing effects of monoterpenoid stylosin in vitro. Fitoterapia 82:742–749
Rassouli FB, Matin MM, Iranshahi M, Bahrami AR, Behravan J, Mollazadeh S, Neshati V, Kalalinia F (2011b) Investigating the enhancement of cisplatin cytotoxicity on 5637 cells by combination with mogoltacin. Toxicol in Vitro 25:469–474
Reichling J, Schnitzler P, Suschke U, Saller R (2010) Essential oils of aromatic plants with antibacterial, antifungal, antiviral and cytotoxic properties. Forsch Komplementmed 16:79–90
Saei-Dehkordi SS, Tajik H, Moradi M, Khalighi-Sigaroodi F (2010) Chemical composition of essential oils in Zataria multiflora Boiss. from different parts of Iran and their radical scavenging and antimicrobial activity. Food Chem Toxicol 48:1562–1567
Solorzano-Santos F, Miranda-Novales MG (2011) Essential oils from aromatic herbs as antimicrobial agents. Curr Opin Biotechnol 23:136–141
Sze SC, Tong Y, Ng TB, Cheng CL, Cheung HP (2010) Herba Epimedii: anti-oxidative properties and its medical implications. Molecules 15:7861–7870
Topcu O, Sumer Z, Tuncer E, Aydin C, Koyuncu A (2009) Efficacy of chlorhexidine gluconate during surgery for hydatid cyst. World J Surg 33:1274–1280
Yang Y, Yue Y, Runwei Y, Guolin Z (2010) Cytotoxic, apoptotic and antioxidant activity of the essential oil of Amomum tsao-ko. Bioresour Technol 101:4205–4211
Yin QH, Yan FX, Zu XY, Wu YH, Wu XP, Liao MC, Deng SW, Yin LL, Zhuang YZ (2011) Anti-proliferative and pro-apoptotic effect of carvacrol on human hepatocellular carcinoma cell line HepG-2. Cytotechnology 64:43–51
Zargari A (1993) Herbal drugs, 6th edn. University of Tehran, Tehran, pp 2–38
Zeghir-Bouteldja R, Amri M, Aitaissa S, Bouaziz S, Mezioug D, Touil-Boukoffa C (2009) In vitro study of nitric oxide metabolites effects on human hydatid of Echinococcus granulosus. J Parasitol Res 2009:624919
Zhang Y, Kim KH, Zhang W, Guo Y, Kim SH, Lu J (2012) Galbanic acid decreases androgen receptor abundance and signaling and induces G1 arrest in prostate cancer cells. Int J Cancer 130:200–212
Zomorodian K, Saharkhiz MJ, Rahimi MJ, Bandegi A, Shekarkhar G, Bandegani A, Pakshir K, Bazargani A (2011) Chemical composition and antimicrobial activities of the essential oils from three ecotypes of Zataria multiflora. Pharm Mag 7:53–59
Acknowledgments
This work was supported by the financial supports from Shiraz University (grant no. 88-GR-AGRST-108). The authors would like to thank their colleagues at the Plants and Drug Research Institute, University of Shahid Beheshti, for their assistance with GC-MS analysis.
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Kavoosi, G., Purfard, A.M. Scolicidal effectiveness of essential oil from Zataria multiflora and Ferula assafoetida: disparity between phenolic monoterpenes and disulphide compounds. Comp Clin Pathol 22, 999–1005 (2013). https://doi.org/10.1007/s00580-012-1518-2
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DOI: https://doi.org/10.1007/s00580-012-1518-2