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Definition of subtypes in the European genotype of porcine reproductive and respiratory syndrome virus: nucleocapsid characteristics and geographical distribution in Europe

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Abstract

The nucleocapsid protein of the European genotype of porcine reproductive and respiratory syndrome virus (type 1, PRRSV-1) exhibited extensive size polymorphism (124–130 amino acids), correlating with phylogenetic grouping of ORF7 as well as ORF5 nucleotide sequences, thereby validating ORF7 size as an independent PRRSV-1 subtype marker. Based on new sequence information from the Russian Federation, we propose division of European genotype PRRSV-1 into 3 subtypes: a pan-European subtype 1 and East European subtypes 2 and 3, with nucleocapsid protein sizes of 128, 125 and 124 amino acids, respectively. The genetic differences between European genotype PRRSV subtypes affected diagnostic RT-PCR primer binding sites. Using Escherichia coli-expressed ORF7 protein, we confirmed that even the relatively closely related PRRSV subtypes 2 and 3 were antigenically different. Finally, the isoelectric point (pI) correlated with the nucleocapsid protein size for European genotype PRRSV subtypes, suggesting subtype-specific compensatory structural changes associated with subtype-specific ORF7 sizes. Thus, the new ORF7-based subtype division of PRRSV-1 proposed here is biologically meaningful and practically relevant.

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Authors and Affiliations

  1. National Veterinary Research Institute, Department of Swine Diseases, OIE Reference Laboratory for PRRS, Partyzantów 57, 24-100, Puławy, Poland

    Tomasz Stadejek & Katarzyna Chabros

  2. Novo Nordisk A/S, Molecular Toxicology, Måløv, Denmark

    Martin B. Oleksiewicz & Jonas S. Krabbe

  3. Federal Centre for Animal Health (FGI “ARRIAH”), OIE Collaborating Center for Diagnosis and Control of Animal Diseases in Eastern Europe, Central Asia and Transcaucasia, 600901, Yur’evets, Vladimir, Russia

    Alexei V. Scherbakov & Anna M. Timina

  4. S.N. Vyshelesskij Institute of Experimental Veterinary Institute, National Academy of Sciences of Belarus, Minsk, Belarus

    Denis Potapchuk

Authors
  1. Tomasz Stadejek
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  2. Martin B. Oleksiewicz
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  3. Alexei V. Scherbakov
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  4. Anna M. Timina
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  5. Jonas S. Krabbe
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  6. Katarzyna Chabros
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  7. Denis Potapchuk
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Correspondence to Tomasz Stadejek.

Additional information

T. Stadejek, M. B. Oleksiewicz and A. V. Scherbakov have contributed equally to this work.

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Stadejek, T., Oleksiewicz, M.B., Scherbakov, A.V. et al. Definition of subtypes in the European genotype of porcine reproductive and respiratory syndrome virus: nucleocapsid characteristics and geographical distribution in Europe. Arch Virol 153, 1479–1488 (2008). https://doi.org/10.1007/s00705-008-0146-2

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  • DOI: https://doi.org/10.1007/s00705-008-0146-2

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