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The effect of taurine on renal ischemia/reperfusion injury

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Summary.

Ischemia-reperfusion (I/R) injury is one of the most common causes of renal dysfunction. Taurine is an endogenous antioxidant and a membrane-stabilizing, intracellular, free beta-amino acid. It has been demonstrated to have protective effects against I/R injuries to tissues other than kidney. The aim of this study was to determine whether taurine has a beneficial role in renal I/R injury. Forty Wistar-Albino rats were allocated into four groups as follows: sham, taurine, I/R, and I/R + taurine. Taurine 7.5 mg/kg was given intra-peritoneally to rats in the groups taurine and I/R + taurine. Renal I/R was achieved by occluding the renal arteries bilaterally for 40 min, followed by 6 h of reperfusion. Immediately thereafter, blood was drawn and tissue samples were harvested to measure 1) serum levels of BUN and creatinine; 2) serum and/or tissue levels of malondialdehyde (MDA), glutathione (GSH), glucose 6-phosphate dehydrogenase (G-6PD), 6-phosphogluconate dehydrogenase (6-PGD) and glutathione reductase (GSH-red); 3) renal morphology; and 4) immunohistochemical staining for P-selectin. Taurine administration reduced I/R-induced increases in serum BUN and creatinine, and serum and tissue MDA levels (p < 0.05). Additionally, taurine lessened the reductions in serum and tissue glutathione levels secondary to I/R (p < 0.05). Taurine also attenuated histopathologic evidence of renal injury, and reduced I/R-induced P-selectin immunoreactivity (p < 0.05). Overall, then, taurine administration appears to reduce the injurious effects of I/R on kidney.

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Authors and Affiliations

  1. Department of Nephrology, Gazi University Faculty of Medicine, Ankara, Turkey

    G. Guz

  2. Department of Physiology, Gazi University Faculty of Medicine, Ankara, Turkey

    E. Oz

  3. Department of Histology and Embryology, Gazi University Faculty of Medicine, Ankara, Turkey

    N. Lortlar & S. Omeroglu

  4. Department of Biochemistry, Hacettpe University Faculty of Medicine, Ankara, Turkey

    N. N. Ulusu

  5. Department of Biochemistry, Gazi University Faculty of Medicine, Ankara, Turkey

    N. Nurlu

  6. Department of Pediatric Surgery, Gazi University Faculty of Medicine, Ankara, Turkey

    B. Demirogullari

  7. Department of Transplantation Surgery, Gazi University Faculty of Medicine, Ankara, Turkey

    S. Sert

  8. Department of Medical Pharmacology, Gazi University Faculty of Medicine, Ankara, Turkey

    C. Karasu

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  1. G. Guz
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Guz, G., Oz, E., Lortlar, N. et al. The effect of taurine on renal ischemia/reperfusion injury. Amino Acids 32, 405–411 (2007). https://doi.org/10.1007/s00726-006-0383-1

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