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Rapid identification of the α-glucosidase inhibitory compounds from Thunberg’s Geranium (Geranium thunbergii Sieb. et Zucc.)

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Abstract

In the current study, our investigation of inhibitory compounds from the ethyl acetate-soluble fraction of 95% ethanol extract of Thunberg’s Geranium (Geranium thunbergii Sieb. et Zucc.; TG) was followed by identification of the inhibitory compounds by a combination of HPLC microfractionation and an enzyme assay in a 96-well plate. Structural analyses of the active compounds were carried out using LC-MSn. The main compound inhibiting yeast α-glucosidase in this plant was tentatively identified as geraniin by LC-MSn. Six compounds, p-hydroxybenzoic acid (1) (IC50 >362.32 μM), brevifolin carboxylic acid (2) (IC50 > 171.23 μM), geraniin (3) (IC50 =4.09 μM), ellagic acid (4) (IC50 = 63.91 μM), kaempferol-3-O-arabinofuranosyl-7-O-rhamnopyranoside (5) (IC50 =27.70 μM), and kaempferitrin (6) (IC50 > 86.51 μM) were isolated from the active EtOAc-soluble fraction of TG on yeast α-glucosidase. However, the 6 isolated compounds were very weak inhibitory activity against mammalian α-glucosidase and α-amylase. This study suggests that the developed HPLC microfractionation with an enzyme assay system is one of the powerful tools for rapid screening and identification of enzyme inhibitors in complex extracts.

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Authors and Affiliations

  1. Department of Food Science and Nutrition, Hallym University, Chuncheon, Gangwon, 200-702, Korea

    Se Jin Choi & Soon Sung Lim

  2. Institute of Natural Medicine, Hallym University, Chuncheon, Gangwon, 200-702, Korea

    Jin Kyu Kim, Jai Man Jang & Soon Sung Lim

  3. Korea Institute of Science and Technology Information, Seoul, 130-741, Korea

    Kuk Hyun Shin

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  1. Se Jin Choi
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Correspondence to Soon Sung Lim.

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Choi, S.J., Kim, J.K., Jang, J.M. et al. Rapid identification of the α-glucosidase inhibitory compounds from Thunberg’s Geranium (Geranium thunbergii Sieb. et Zucc.). Food Sci Biotechnol 21, 987–996 (2012). https://doi.org/10.1007/s10068-012-0129-7

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  • DOI: https://doi.org/10.1007/s10068-012-0129-7

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