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Low-level red laser improves healing of second-degree burn when applied during proliferative phase

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Abstract

The aim of this study was to investigate the effects of low-level red laser on tissue repair in rats submitted to second-degree burn, evaluating if the timing of laser treatment influences the healing process. The animals had their backs shaved and divided as follows: control group (n = 12)—rats burned and not irradiated, early laser group (n = 12)—rats burned and irradiated from day 1 after injury for five consecutive days, and late laser group (n = 14)—rats burned and irradiated from day 4 after injury for five consecutive days. Laser irradiation was according to a clinical protocol (20 J/cm2, 100 mW, continuous wave emission mode, 660 nm) as recommended by the laser device manufacturer. Half of the animals were sacrificed 10 days after burn, and the other animals were sacrificed 21 days after burn. The late laser group accelerated wound contraction 10 and 21 days after burn. The late laser group accelerated reepithelialization 18 days after burn. The late laser group increases the granulation tissue 10 and 21 days after burn. Both irradiated groups increased type III collagen expression and TGF-β 21 days after burn. Both irradiated groups increased macrophage and myofibroblast numbers 10 days after burn and decreased 21 days after. Low-level red laser exposure contributes to the process of tissue repair of second-degree burns, but the intervention during proliferative phase is crucial in the final outcome of the repair process.

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Authors and Affiliations

  1. Departamento de Histologia e Embriologia (DHE), Universidade do Estado do Rio de Janeiro (UERJ), Av Marechal Rondon 381/HLA, Rio de Janeiro, RJ, 20950-003, Brazil

    Eduardo Tavares Lima Trajano, Larissa Alexsandra da Trajano, Marco Aurélio dos Santos Silva, Luís Cristóvão de Porto & Andréa Monte-Alto-Costa

  2. Departamento de Cirurgia Geral (DCG), Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro, Brazil

    Neil Grant Venter

  3. Departamento de Biofísica e Biometria (DBB), Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro, Brazil

    Adenilson de Fonseca

Authors
  1. Eduardo Tavares Lima Trajano
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  2. Larissa Alexsandra da Trajano
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  3. Marco Aurélio dos Santos Silva
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  4. Neil Grant Venter
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  5. Luís Cristóvão de Porto
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  6. Adenilson de Fonseca
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  7. Andréa Monte-Alto-Costa
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Correspondence to Andréa Monte-Alto-Costa.

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Trajano, E.T.L., da Trajano, L.A., dos Santos Silva, M.A. et al. Low-level red laser improves healing of second-degree burn when applied during proliferative phase. Lasers Med Sci 30, 1297–1304 (2015). https://doi.org/10.1007/s10103-015-1729-2

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  • DOI: https://doi.org/10.1007/s10103-015-1729-2

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