Abstract
Distribution of Cucumber mosaic virus (CMV) in shoot meristem tissue of CMV-inoculated tobacco was successively analyzed with immunohistochemical microscopy and in situ hybridization. CMV signals were detected in the tissue at 7 days postinoculation (dpi), but then they decreased and disappeared after 14 dpi. Detailed observation confirmed CMV invasion of shoot apical meristem at 6–8 dpi. Short interfering RNA corresponding to CMV RNAs was first detected at 7 dpi and was detected up to 24 dpi. These results suggest that the shoot meristem tissue is infected with CMV but subsequently recovers from the infection by RNA silencing.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Mochizuki, T., Ohki, S. Shoot meristem tissue of tobacco inoculated with Cucumber mosaic virus is infected with the virus and subsequently recovers from infection by RNA silencing. J Gen Plant Pathol 70, 363–366 (2004). https://doi.org/10.1007/s10327-004-0140-9
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10327-004-0140-9