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First report of Citrus viroid V in Japan

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Abstract

We tested citrus samples from Japan using reverse transcription-polymerase chain reaction (RT-PCR), and for the first time, Citrus viroid V (CVd-V) was detected in Japan. Using nucleotide sequence analysis, we identified two new CVd-V variants, which shared 91–96% nucleotide sequence identities with those reported previously and 89% with each other. The RT-PCR detected CVd-V from 44 of 275 citrus trees tested, showing that CVd-V can be spread to some extent within commercial orchards in Japan.

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Acknowledgments

We thank all those who provided us with samples for this study and assisted in their collection.

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Authors and Affiliations

  1. Grape and Persimmon Research Station, National Institute of Fruit Tree Science (NIFTS), Akitsu, Hiroshima, 739-2494, Japan

    Takao Ito

  2. Okitsu Citrus Research Station, NIFTS, Okitsu, Shizuoka, Japan

    Satoshi Ohta

Authors
  1. Takao Ito
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  2. Satoshi Ohta
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Corresponding author

Correspondence to Takao Ito.

Additional information

Contribution No. 1564 of NIFTS. The nucleotide sequence data of the Citrus viroid V (CVd-V) variants reported are available in the DDBJ/EMBL/GenBank databases as accessions AB560862 and AB560863.

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Ito, T., Ohta, S. First report of Citrus viroid V in Japan. J Gen Plant Pathol 76, 348–350 (2010). https://doi.org/10.1007/s10327-010-0254-1

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  • DOI: https://doi.org/10.1007/s10327-010-0254-1

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