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A broad spectrum, one-step RT-PCR to detect Satsuma dwarf virus variants using universal primers targeting both segmented RNAs 1 and 2

  • Viral and Viroid Diseases
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An Erratum to this article was published on 07 February 2012

Abstract

Universal primers to detect Satsuma dwarf virus (SDV), including distantly related strains Citrus mosaic virus (CiMV), Navel orange infectious mottling virus (NIMV), and Hyuganatsu virus (HV), were tested in a convenient one-step RT-PCR assay. SDV was the most broadly detected using uSDVup/uSDVdo primers that specifically targeted a nucleotide sequence in the 3′-noncoding region that is conserved in both segmented RNAs 1 and 2 of SDV among the tested primers. Nucleotide sequence analysis confirmed that the amplified RT-PCR products could be derived from RNAs 1 or 2 of SDV variants, some of which had interesting genetic diversity.

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References

  • Ito T, Iwanami T, Ieki H, Shimomura K, Shimizu S, Ito T (2004) A new virus related to Satsuma dwarf virus: the nucleotide sequence of the 3′-terminal regions of Hyuganatsu virus RNAs 1 and 2. Arch Virol 149:1459–1465

    Article  PubMed  CAS  Google Scholar 

  • Ito T, Ito T, Shiotani H, Iwanami T, Ozaki K, Muramoto K (2007) Genetic diversity and a heterogeneous population of Citrus mosaic virus within a single citrus tree. J Gen Plant Pathol 73:147–151

    Article  CAS  Google Scholar 

  • Iwanami T (2010) Properties and control of Satsuma dwarf virus. JARQ 44:1–6

    Article  CAS  Google Scholar 

  • Iwanami T, Ieki H (1996) Nucleotide sequence of the 3′-terminal region of citrus mosaic virus RNA1. In: da Graça JV, Moreno P, Yokomi RK (eds) Proceedings of the 13th conference of the international organization of citrus virologists. IOCV, Riverside, pp 201–207

    Google Scholar 

  • Le Gall O, Iwanami T, Karasev AV, Jones T, Lehto K, Sanfaçon H, Wellink J, Wetzel T, Yoshikawa N (2005) Genus Sadwavirus. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy: eighth report of the international committee on taxonomy of viruses. Academic Press, San Diego, pp 799–802

    Google Scholar 

  • Nakaune R, Nakano M (2006) Efficient methods for sample processing and cDNA synthesis by RT-PCR for the detection of grapevine viruses and viroids. J Virol Methods 134:244–249

    Article  PubMed  CAS  Google Scholar 

  • Thompson JR, Jelkmann W (2003) The detection and variation of Strawberry mottle virus. Plant Dis 87:385–390

    Article  CAS  Google Scholar 

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Acknowledgments

The authors thank those who provided us with the samples and assisted us with collections. The authors also thank Dr. Nakaune for technical advice on the sandpaper method.

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Author notes

  1. Shin-ichi Shimizu

    Present address: Ehime Prefectural Government, Matsuyama, Ehime, Japan

  2. Takanori Miyoshi

    Present address: Ehime Prefectural Toyo Regional Office Imabari Bureau, Imabari, Ehime, Japan

  3. Yasunobu Tachibana

    Present address: Ehime Agricultural College, Matsuyama, Ehime, Japan

Authors and Affiliations

  1. Fruit Tree Research Center, Ehime Research Institute of Agriculture, Forestry and Fisheries, Matsuyama, Ehime, Japan

    Shin-ichi Shimizu, Takanori Miyoshi & Yasunobu Tachibana

  2. Grape and Persimmon Research Station, National Institute of Fruit Tree Science (NIFTS), Akitsu, Hiroshima, 739-2494, Japan

    Takao Ito

  3. Apple Research Station, NIFTS, Iwate, Japan

    Tsutae Ito

Authors
  1. Shin-ichi Shimizu
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  2. Takao Ito
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  3. Takanori Miyoshi
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  4. Yasunobu Tachibana
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  5. Tsutae Ito
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Corresponding author

Correspondence to Takao Ito.

Additional information

The nucleotide sequence data of the Satsuma dwarf virus clones 1Gd, 1Gg, and 8Da are available in the DDBJ/EMBL/GenBank databases as accessions AB639137–AB639139.

An erratum to this article can be found at http://dx.doi.org/10.1007/s10327-011-0358-2.

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Shimizu, Si., Ito, T., Miyoshi, T. et al. A broad spectrum, one-step RT-PCR to detect Satsuma dwarf virus variants using universal primers targeting both segmented RNAs 1 and 2. J Gen Plant Pathol 77, 326–330 (2011). https://doi.org/10.1007/s10327-011-0342-x

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  • DOI: https://doi.org/10.1007/s10327-011-0342-x

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