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Determination and Distribution Study of Myrislignan in Rat Tissues by RP-HPLC

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Abstract

Myrislignan (MRL) existing in nutmeg has been reported to possess inhibition of neoplasm and vascular smooth muscle contraction, and cause a prolonging of hexobarbital-induced hypnosis in mice. In this study, a high-performance liquid chromatography–ultraviolet detection (HPLC–UV) method was developed to analyze MRL in rat tissues. The separation was carried out on an analytical Diamonsil™ ODS C18 HPLC column (250 × 4.6 mm, 5 μm) equipped with a C18 guard column (8 × 4 mm, 5 μm) cartridge system eluted at 1.0 mL min−1 with a mobile phase methanol–H2O (3:1, v/v). Detection wavelength was 270 nm. The concentration of MRL was analyzed in the brain, testes, muscle, adipose, kidney, heart, lung, stomach, intestine, liver, and spleen at various time points between 8 and 128 min following intravenous administration of MRL to rats at a dose of 20.0 mg kg−1. The method was successfully used to study the tissue distribution of MRL. The results contribute to explain the effect of nutmeg on the brain, liver and gastrointestinal tract.

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Acknowledgments

The authors thank the NIH Fellows Editorial Board (NIH, Bethesda) for editorial assistance. This research was supported partly by the National Natural Science Foundation of China (30973863; 81161120429), National Key Technology Research and Development Program of China (2011BAI07B08), and Beijing Municipal Special–Purpose Science Foundation of China (Z0004105040311).

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Author notes

  1. Fei Li

    Present address: Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 37, Room 3112, Bethesda, MD, 20892, USA

Authors and Affiliations

  1. State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Haidian District, 100191, Beijing, China

    Ying Wang, Jia-Xi Liu, You-Bo Zhang, Fei Li & Xiu-Wei Yang

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  1. Ying Wang
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  2. Jia-Xi Liu
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Correspondence to Xiu-Wei Yang.

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Wang, Y., Liu, JX., Zhang, YB. et al. Determination and Distribution Study of Myrislignan in Rat Tissues by RP-HPLC. Chromatographia 75, 541–549 (2012). https://doi.org/10.1007/s10337-012-2219-3

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  • DOI: https://doi.org/10.1007/s10337-012-2219-3

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