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Frederiksenia canicola gen. nov., sp. nov. isolated from dogs and human dog-bite wounds

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Abstract

Polyphasic analysis was done on 24 strains of Bisgaard taxon 16 from five European countries and mainly isolated from dogs and human dog-bite wounds. The isolates represented a phenotypically and genetically homogenous group within the family Pasteurellaceae. Their phenotypic profile was similar to members of the genus Pasteurella. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry clearly identified taxon 16 and separated it from all other genera of Pasteurellaceae showing a characteristic peak combination. Taxon 16 can be further separated and identified by a RecN protein signature sequence detectable by a specific PCR. In all phylogenetic analyses based on 16S rRNA, rpoB, infB and recN genes, taxon 16 formed a monophyletic branch with intraspecies sequence similarity of at least 99.1, 90.8, 96.8 and 97.2 %, respectively. Taxon 16 showed closest genetic relationship with Bibersteinia trehalosi as to the 16S rRNA gene (95.9 %), the rpoB (89.8 %) and the recN (74.4 %), and with Actinobacillus lignieresii for infB (84.9 %). Predicted genome similarity values based on the recN gene sequences between taxon 16 isolates and the type strains of known genera of Pasteurellaceae were below the genus level. Major whole cell fatty acids for the strain HPA 21T are C14:0, C16:0, C18:0 and C16:1 ω7c/C15:0 iso 2OH. Major respiratory quinones are menaquinone-8, ubiquinone-8 and demethylmenaquinone-8. We propose to classify these organisms as a novel genus and species within the family of Pasteurellaceae named Frederiksenia canicola gen. nov., sp. nov. The type strain is HPA 21T (= CCUG 62410T = DSM 25797T).

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Acknowledgments

We thank Geoffrey Foster for sending strain M2500/96/3 and Andreas Thomann for technical assistance.

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Authors and Affiliations

  1. Vetsuisse Faculty, Institute of Veterinary Bacteriology, University of Bern, Laenggass-Strasse 122, 3001, Bern, Switzerland

    Bożena M. Korczak & Peter Kuhnert

  2. Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, 4 Stigbøjlen, 1870, Frederiksberg C, Denmark

    Henrik Christensen

  3. Horsevaenget 40, 4130, Viby Sjælland, Denmark

    Magne Bisgaard

Authors
  1. Bożena M. Korczak
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  2. Magne Bisgaard
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  3. Henrik Christensen
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  4. Peter Kuhnert
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Correspondence to Peter Kuhnert.

Electronic supplementary material

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10482_2014_129_MOESM1_ESM.pdf

Comparison of mass spectra profiles of Frederiksenia canicola and type species of currently validated genera. Peak locations and their relative intensities are indicated. Spectra were taken from the Biotyper database V.3 (indicated by an asterisk) and from Kuhnert P, Bisgaard M, Korczak BM, Schwendener S, Christensen H, Frey J (2012) Identification of animal Pasteurellaceae by MALDI-TOF mass spectrometry. J Microbiol Methods 89:1–7 (PDF 85 kb)

10482_2014_129_MOESM2_ESM.pdf

Phylogenetic relationship of Frederiksenia canicola and other members of the family Pasteurellaceae based on Neighbor Joining tree of partial rpoB sequences. Escherichia coli was included as an outgroup to root the dendrogram. The clustering was supported by cophenetic correlation. The scale bar indicates sequence differences between the taxa. *Gene sequence ID, Oralgen www.oralgen.lanl.gov (PDF 82 kb)

10482_2014_129_MOESM3_ESM.pdf

Phylogenetic relationship of Frederiksenia canicola and other members of the family Pasteurellaceae based on Neighbor Joining tree of partial infB sequences. The clustering was supported by cophenetic correlation. The scale bar indicates sequence differences between the taxa. *Gene sequence ID, Oralgen www.oralgen.lanl.gov (PDF 82 kb)

10482_2014_129_MOESM4_ESM.pdf

Phylogenetic relationship of Frederiksenia canicola and other members of the family Pasteurellaceae based on Neighbor Joining tree of partial recN sequences. Escherichia coli was included as an outgroup to root the dendrogram. The clustering was supported by cophenetic correlation. The scale bar indicates sequence differences between the taxa. *Gene sequence ID, Oralgen www.oralgen.lanl.gov (PDF 81 kb)

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Korczak, B.M., Bisgaard, M., Christensen, H. et al. Frederiksenia canicola gen. nov., sp. nov. isolated from dogs and human dog-bite wounds. Antonie van Leeuwenhoek 105, 731–741 (2014). https://doi.org/10.1007/s10482-014-0129-0

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