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Erysipelothrix larvae sp. nov., isolated from the larval gut of the rhinoceros beetle, Trypoxylus dichotomus (Coleoptera: Scarabaeidae)

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An Erratum to this article was published on 25 November 2015

Abstract

A novel, Gram-stain positive, facultative anaerobic, non-motile and straight to curve rod shaped bacterium, strain LV19T was isolated from the larval gut of the rhinoceros beetle, Trypoxylus dichotomus, which was collected from Yeong-dong, Chuncheongbuk-do, South Korea. The colonies of the new isolate were convex, circular, cream white in color and 1–2 mm in diameter after 3 days incubation on Tryptic Soy Agar at 37 °C. Based on the 16S rRNA gene sequence similarity, the new isolate was most closely related to Erysipelothrix inopinata MF-EP02T, E. rhusiopathiae ATCC 19414 T and E. tonsillarum T-305T (94.8, 93.8 and 93.7 % similarity, respectively). Strain LV19T grew optimally at 37 °C, at pH 8.0 and in the presence of 0.5 % (w/v) NaCl. Oxidase activity and catalase activity were negative. The major cellular fatty acids (>10 %) were C18:2 cis-9,12 (28.9 %), C18:1 cis-9 (22.3 %), C16:0 (22.2 %) and C18:0 (18.5 %). The cell-wall hydrolysates contained ribose as a major sugar. Major polar lipids were phosphatidylglycerol and three unidentified glycolipids. No quinone was detected. The G+C content of the genomic DNA was 36.3 mol%. The levels of DNA–DNA relatedness between strain LV19T and all the reference strains were less than 20 %. On the basis of polyphasic evidence from this study, the isolate is considered to represent a novel species of the genus Erysipelothrix, for which the name Erysipelothrix larvae sp. nov. is proposed; the type strain is LV19T (=KCTC 33523T = DSM 28480T).

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Abbreviations

PG:

Phosphatidylglycerol

GL:

Unidentified glycolipid

PL:

Unidentified phospholipid,

AL:

Unidentified aminolipid,

APGL:

Unidentified aminophosphoglycolipid

TSA:

Tryptic Soy Agar

rpm:

Revolution per minute

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Acknowledgments

This work was supported by the Research Program for Agricultural Science & Technology Development (Project No. PJ010168) and was partially supported by grants from the National Research Foundation of Korea (NRF) (2008-2004721), the KRIBB Research Initiative Programs (KGM4111443 & KCM1051413), and by a grant from of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (Grant Number: HI14C 03680200).

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  1. Korean Collection for Type Cultures (KCTC), Biological Resource Center (BRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-gu, Daejeon, 305-806, Republic of Korea

    Moon-Soo Rhee, Dong-Ho Chang, Doo-Sang Park & Byoung-Chan Kim

  2. Department of Food and Nutrition Science, Eulji University, 553 Sanseong-Daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, 461-832, Republic of Korea

    Byung-Ho Bang

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Correspondence to Byoung-Chan Kim.

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Bang, BH., Rhee, MS., Chang, DH. et al. Erysipelothrix larvae sp. nov., isolated from the larval gut of the rhinoceros beetle, Trypoxylus dichotomus (Coleoptera: Scarabaeidae). Antonie van Leeuwenhoek 107, 443–451 (2015). https://doi.org/10.1007/s10482-014-0342-x

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