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Ginsenoside-Rd potentiates apoptosis induced by hydrogen peroxide in basilar artery smooth muscle cells through the mitochondrial pathway

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Abstract

Our previous studies showed that ginsenoside-Rd, a purified component from Panax notoginseng, inhibited cell proliferation and reversed basilar artery remodeling. The aim of this study was to investigate whether ginsenoside- Rd influences H2O2-induced apoptosis in basilar artery smooth muscle cells (BASMCs). The results showed that ginsenoside-Rd significantly potentiated H2O2-induced cell death and cell apoptosis. This resulted in a concentration-dependent reduction of the cell viability. Ginsenoside-Rd further increased cytochrome C release and caspase-9/caspase-3 activations, and reduced the stability of mitochondrial membrane potential (MMP) and the ratio of Bcl-2/Bax. Cyclosporine A, an inhibitor of mitochondrial-permeability transition, inhibited alteration of mitochondrial permeability induced by H2O2 and reversed the effect of ginsenoside-Rd on MMP. Our data strongly suggest that ginsenoside-Rd potentiated H2O2-induced apoptosis of BASMCs through the mitochondria-dependent pathway.

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Acknowledgments

This work was supported by the National Basic Research Program of China (973 Program; grant No. 2009CB521903), National Nature Science Foundation of China (key grant No. 30730105), and Science and Technology Planning Project of Guangdong Province, China (2011B080701012).

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Authors and Affiliations

  1. Department of Pharmacology, Cardiac and Cerebrovascular Research Center, 510080, Guangzhou, People’s Republic of China

    Shi-Yang Li, Ming-Ming Ma, Yun Liu, Yan-Hua Du, Xiao-Fei Lv, Jia-Guo Zhou, Yong-Bo Tang & Yong-Yuan Guan

  2. Department of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-Sen University, 510080, Guangzhou, People’s Republic of China

    Xiao-Guang Wang

  3. Department of Pharmacology, Cardiac and Cerebrovascular Research Center, Zhongshan School of Medcine, Sun Yat-Sen University, 74 Zhongshan 2 Rd, 510080, Guangzhou, China

    Yong-Bo Tang & Yong-Yuan Guan

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  1. Shi-Yang Li
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  2. Xiao-Guang Wang
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  4. Yun Liu
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Correspondence to Yong-Bo Tang or Yong-Yuan Guan.

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Shi-Yang Li, Xiao-Guang Wang authors contributed equally to this work.

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Li, SY., Wang, XG., Ma, MM. et al. Ginsenoside-Rd potentiates apoptosis induced by hydrogen peroxide in basilar artery smooth muscle cells through the mitochondrial pathway. Apoptosis 17, 113–120 (2012). https://doi.org/10.1007/s10495-011-0671-4

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