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17β-Estradiol protects against apoptosis induced by levofloxacin in rat nucleus pulposus cells by upregulating integrin α2β1

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Abstract

Levofloxacin has been reported to have cytotoxicity to chondrocytes in vitro. And 17β-estradiol has been widely studied for its protective effects against cell apoptosis. Based on apoptotic cell model induced by levofloxacin, the purpose of this study was to explore the mechanism by which 17β-estradiol protects rat nucleus pulposus cells from apoptosis. Inverted phase-contrast microscopy, flow cytometry, and caspase-3 activity assay were used to find that levofloxacin induced marked apoptosis, which was abolished by 17β-estradiol. Interestingly, estrogen receptor antagonist, ICI182780, and functional blocking antibody to α2β1 integrin, both prohibited the effect of 17β-estradiol. Simultaneously, levofloxacin decreased cellular binding ability to type II collagen, which was also reversed by 17β-estradiol. Furthermore, western blot and real-time quantitative PCR were used to find that integrin α2β1 was responsible for estrogen-dependent anti-apoptosis, which was time–response and dose–response effect. 17β-estradiol was proved for the first time to protect rat nucleus pulposus cells against levofloxacin-induced apoptosis by upregulating integrin α2β1 signal pathway.

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Authors and Affiliations

  1. Department of Spine Surgery, The Third Hospital of Hebei Medical University, NO.139 Ziqiang Road, Shijiazhuang, 050051, China

    Si-Dong Yang, Lei Ma, Wen-Yuan Ding, Yong Shen, Ying-Ze Zhang, Da-Long Yang, Di Zhang, Ya-Peng Sun & Yan-Li Song

  2. Department of VIP Ward, The First Hospital of Hebei Medical University, Shijiazhuang, 050051, China

    Ti-Xin Gu

  3. Department of Rehabilitation Medicine, The Third Hospital of Hebei Medical University, Shijiazhuang, 050051, China

    Feng Zhang

  4. Hebei Provincial Key Laboratory of Orthopedic Biomechanics, Shijiazhuang, 050051, China

    Si-Dong Yang, Lei Ma, Wen-Yuan Ding, Yong Shen, Ying-Ze Zhang, Da-Long Yang, Di Zhang & Ya-Peng Sun

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  1. Si-Dong Yang
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Correspondence to Wen-Yuan Ding.

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Si-Dong Yang and Lei Ma have contributed equally to this study.

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Yang, SD., Ma, L., Gu, TX. et al. 17β-Estradiol protects against apoptosis induced by levofloxacin in rat nucleus pulposus cells by upregulating integrin α2β1 . Apoptosis 19, 789–800 (2014). https://doi.org/10.1007/s10495-014-0965-4

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