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Resveratrol protects rabbit articular chondrocyte against sodium nitroprusside-induced apoptosis via scavenging ROS

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Abstract

This study aims to investigate the mechanism by which resveratrol (RV) prevents sodium nitroprusside (SNP)-induced chondrocyte apoptosis, which is a characteristic feature of osteoarthritis (OA). Rabbit articular chondrocytes were pre-incubated with 100 μM RV for 18 h before 1.5 mM SNP co-treatment for 6 h. Cell viability was evaluated by CCK-8. Annexin V/PI double staining and Hoechst 33258 staining were used to determine the fashion of SNP-induced chondrocytes death. Mitochondrial membrane potential (ΔΨm) was measured by using flow cytometry (FCM) with TMRM and Rhodamine 123 staining. Intracellular reactive oxygen species (ROS) and nitric oxide (NO) levels were confirmed by FCM analysis with DCFH-DA and DAF-FM DA staining. Cytoskeleton proteins of chondrocytes co-stained with Actin-Trakcer Green and Tubulin-Trakcer Red were validated by confocal microscopy. SNP induced time- and dose-dependent chondrocytes apoptosis with decline of ΔΨm, activation of caspases as well as cytoskeletal remodeling. SNP induced a significant induction of both ROS and NO. RV remarkably prevented SNP-induced ROS production and apoptosis as well as cytoskeletal remodeling, but did not prevent SNP-induced NO production. Pretreatment with NO scavengers did not significantly prevent SNP-induced apoptosis and cytoskeletal remodeling. SNP induces NO-independent ROS production which dominates rabbit articular chondrocyte apoptosis, and RV protects chondrocytes against SNP-induced apoptosis via scavenging ROS instead of NO.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (81071491 and 61178078), Key Project of the Department of Education and Finance of Guangdong Province (cxzd115) and the Opening Project of MOE Key laboratory of Laser Life Science, South China Normal University.

Conflict of interest

The authors declare no conflicts of interest.

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Authors and Affiliations

  1. Department of Pain Management, The First Affiliated Hospital of Jinan University, Guangzhou, 510630, China

    Qian Liang & Xiao-ping Wang

  2. MOE Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510630, China

    Tong-sheng Chen

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  1. Qian Liang
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  2. Xiao-ping Wang
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Correspondence to Xiao-ping Wang.

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Liang, Q., Wang, Xp. & Chen, Ts. Resveratrol protects rabbit articular chondrocyte against sodium nitroprusside-induced apoptosis via scavenging ROS. Apoptosis 19, 1354–1363 (2014). https://doi.org/10.1007/s10495-014-1012-1

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  • DOI: https://doi.org/10.1007/s10495-014-1012-1

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