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Effect of triploidization on juvenile African catfish (Clarias gariepinus)

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Abstract

Triploid African catfish (Clarias gariepinus) produced through cold shock 3 min post fertilization were compared to diploid offspring of the same parentage at 66 days of age. Triploid fish were significantly shorter (11%) and weighed less (18%) than diploids but showed no significant difference in mortality or cannibalism, which can be an important source of losses under aquaculture conditions. Erythrocytes of triploid fish were more oval shaped than the normal spherical shape of diploid erythrocytes, were significantly larger (38%) and had nuclei that were significantly larger (25%) than observed in diploid fish. Erythrocyte morphological characteristics show potential as an indicator for the detection of triploidy in African catfish.

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References

  • Adewolu MA, Adeniji CA, Adejobi AB (2008) Feed utilization, growth and survival of Clarias gariepinus (Burchell 1822) fingerlings cultured under different photoperiods. Aquaculture 283:64–67

    Article  Google Scholar 

  • Akinwole AO, Faturoti EO (2007) Biological performance of African Catfish (Clarias gariepinus) cultured in recirculating system in Ibadan. Aquac Eng 36:18–23

    Article  Google Scholar 

  • Al-Fageeh MB, Smales CM (2006) Control and regulation of the cellular responses to cold shock: the responses in yeast and mammalian systems. Biochem J Exp Zool 397:247–259

    CAS  Google Scholar 

  • Appelbaum S, Kamler E (2000) Survival, growth, metabolism and behaviour of Clarias gariepinus (Burchell 1822) early stages under different light conditions. Aquac Eng 22:269–287

    Article  Google Scholar 

  • Baksi SM, Means JC (1988) Preparation of chromosomes from early stages of fish for cytogenetic analysis. J Fish Biol 32:321–325

    Article  Google Scholar 

  • Baur B (1994) Inter-population differences in propensity for egg cannibalismin hatchings of the land snail Arianta arbustorum. Anim Behav 48:851–860

    Article  Google Scholar 

  • Benfey TJ (1999) The physiology and behavior of triploid fishes. Rev Fish Sci 7:39–67

    Article  Google Scholar 

  • Britz PJ, Hecht T (1987) Temperature preferences and optimum temperature for growth of African sharptooth catfish (Clarias gariepinus) larvae and post-larvae. Aquaculture 63:205–214

    Article  Google Scholar 

  • da Silva FSD, Moreira RG, Orozco-Zapata CR, Hilsdorf AWS (2007) Triploidy induction by cold shock in the South American catfish, Rhamdia quelen (Siluriformes) (Quoy & Gaimard, 1824). Aquaculture 272:S110–S114

    Article  Google Scholar 

  • Donaldson EM, Devlin RH (1996) Uses of biotechnology to enhance production. In: Pennell W, Barton B (eds) Principles of salmonid culture. Developments in aquaculture and fisheries science. Elsevier, Amsterdam, pp 969–1020

    Google Scholar 

  • Elgar MA, Crespi BJ (1992) Ecology and evolution of cannibalism. In: Elgar MA, Crespi BJ (eds) Cannibalism. Oxford University Press, Oxford, pp 1–12

    Google Scholar 

  • Espinosa E, Josa A, Gil L, Ignacio Marti J (2005) Triploidy in rainbow trout determined by computer-assisted analysis. J Exp Zool 303:1007–1012

    Article  Google Scholar 

  • Felip A, Piferrer F, Zanuy S, Carrillo M (2001) Comparative growth performance of diploid and triploid European sea bass over the first four spawning seasons. J Fish Biol 58:76–88

    Article  Google Scholar 

  • Flajšhans M, Kvanicka P, Rab P (1993) Genetic studies in tench (Tinca tinca L.): high incidence of spontaneous triploidy. Aquaculture 110:243–248

    Article  Google Scholar 

  • Galbreath PF, Thorgaard GH (1995) Saltwater performance of all-female triploid Atlantic salmon. Aquaculture 138:77–85

    Article  Google Scholar 

  • Gillet C, Vauchez C, Haffray P (2001) Triploidy induced by pressure shock in Arctic charr (Salvelinus alpinus): growth, survival and maturation until the third year. Aquat Living Resour 14:327–334

    Article  Google Scholar 

  • Hecht T, Appelbaum S (1988) Observations on intraspecific agression and coeval sibling cannibalism by larval and juvenile Clarias gariepinus (Clariidae: Pisces) under controlled conditions. J Zool 214:21–44

    Article  Google Scholar 

  • Henken AM, Brunink AM, Richter CJJ (1987) Differences in growth rate and feed utilization between diploid and triploid African catfish, Clarias gariepinus (Burchell 1822). Aquaculture 63:233–242

    Article  Google Scholar 

  • Hildebrandt B, Wust P, Alhers O (2002) The cellular and molecular basis of hyperthermia. Rev Oncol Hematol 43:33–56

    Article  Google Scholar 

  • Johnson JC (2001) Sexual cannibalism in fishing spiders (Dolomedes triton): an evaluation of two explanations for female aggression towards potential mates. Anim Behav 61:905–914

    Article  Google Scholar 

  • Le Curieux-Belfond O, Vandelac L, Caron J, Séralini G-É (2009) Factors to consider before production and commercialization of aquatic genetically modified organisms: the case of transgenic salmon. Environ Sci Policy 12:170–189

    Article  CAS  Google Scholar 

  • Lima NRW, Vrijenhoek RC (1996) Avoidance of filial cannibalism by sexual and clonal forms of Poeciliopsis (Pisces: Poeciliidae). Anim Behav 51:293–301

    Article  Google Scholar 

  • Maxime V (2008) The physiology of triploid fish: current knowledge and comparisons with diploid fish. Fish Fish 9:67–78

    Google Scholar 

  • Miskolczi E, Mihalffy S, Patakine Varkonyi E, Urbanyi B, Horvath A (2005) Examination of larval malformations in African catfish Clarias gariepinus following fertilization with cryopreserved sperm. Aquaculture 247:119–125

    Article  Google Scholar 

  • Mol K, Byamungu N, Cuisset B, Yaron Z, Ofir M, Melard C, Castelli M, Kuhn ER (1994) Hormonal profile of growing male and female diploids and triploids of the blue tilapia, Oreochromis aureus, reared in intensive culture. Fish Physiol Biochem 13:209–218

    Article  CAS  Google Scholar 

  • Najiah M, Nadirah M, Marina H, Lee SW, Nazaha WH (2008) Quantitative comparisons of erythrocyte morphology in healthy freshwater fish species from Malaysia. INSInet 3:32–35

    Google Scholar 

  • Naylor RL, Goldburg RJ, Primavera JH, Kautsky N, Beveridge MCM, Clay J, Folke C, Lubchenco J, Mooney H, Troell M (2000) Effect of aquaculture on world fish supplies. Nature 405:1017–1024

    Article  CAS  PubMed  Google Scholar 

  • Nishimura K, Isoda Y (2004) Evolution of cannibalism: referring to costs of cannibalism. J Theor Biol 226:293–302

    Article  PubMed  Google Scholar 

  • Oppedal F, Taranger GL, Hansen T (2003) Growth performance and sexual maturation in diploid and triploid Atlantic salmon (Salmo salar L.) in seawater tanks exposed to continuous light or simulated natural photoperiod. Aquaculture 215:145–162

    Article  Google Scholar 

  • Peruzzi S, Varsamos S, Chatain B, Fauvel C, Menu B, Falguière JC, Sévère A, Flik G (2005) Haematological and physiological characteristics of diploid and triploid sea bass, Dicentrarchus labrax L. Aquaculture 244:359–367

    Article  Google Scholar 

  • Piferrer F, Cal RM, Álvarez-Blázquez B, Sánchez L, Martinez P (2000) Induction of triploidy in the turbot (Scophthalmus maximus): I. Ploidy determination and the effects of cold shocks. Aquaculture 188:79–90

    Article  Google Scholar 

  • Purdom CE (1972) Induced polyploidy in plaice (Pleuronectes platessa) and its hybrid with the flounder (Platichthys flesus). Heredity 29:11–24

    Article  CAS  PubMed  Google Scholar 

  • Richter CJJ, Henken AM, Eding EH, Van Doesum JH, De Boer P (1986) Induction of triploidy by cold-shocking eggs and performance of triploids in the African catfish, Clarias gariepinus (Burchell 1822). In: EIFAC/FAO symposium on selection, hybridization and genetic engineering in aquaculture of fish and shellfish for consumption and stocking, Bordeaux, p 24

  • Samu F, Toft S, Kiss B (1999) Factors influencing cannibalism in the wolf spider Pardosa agrestis (Araneae, Lycosidae). Behav Ecol Sociobiol 45:349–354

    Article  Google Scholar 

  • Small SA, Benfey TJ (1987) Cell size in triploid salmon. J Exp Zool 241:339–342

    Article  Google Scholar 

  • Stanley JG, Allen SK Jr, Hidu H (1981) Polyploidy induced in the American oyster, Crassostrea virginica, with cytochalasin B. Aquaculture 23:1–10

    Article  CAS  Google Scholar 

  • Streisinger G, Walker C, Dower N (1981) Production of clones of homozygous diploid zebra fish (Brachydanio rerio). Nature 291:293–296

    Article  CAS  PubMed  Google Scholar 

  • Thibault RE (1974) Genetics of cannibalism in a viviparous fish and its relationship to population density. Nature 251:138–140

    Article  Google Scholar 

  • Thorgaard GH, Gall GA (1979a) Adult triploids in a rainbow trout family. Genetics 93:961–973

    CAS  PubMed  Google Scholar 

  • Thorgaard GH, Gall GA (1979b) Adult triploids in a rainbow trout family. Genetics 103:771–783

    Google Scholar 

  • Tiwary BK, Kirubagaran R, Ray AK (1997) Induction of triploidy by cold shock in catfish, Heteropneustes fossilis (Bloch). Asian Fish Sci 10:123–129

    Google Scholar 

  • Tiwary BK, Kirubagaran R, Ray AK (2004) The biology of triploid fish. Rev Fish Biol Fish 14:391–402

    Article  Google Scholar 

  • Utting SD, Child AR (1994) Genetic manipulation of the Manila clam (Tapes philippinarum) using cytochalasin B to induce triploidy. Aquaculture 120:271–282

    Article  Google Scholar 

  • Varadaraj K, Pandian TJ (1988) Induction of triploids in Oreochromis mossambicus by thermal, hydrostatic pressure and chemical shocks. Proceedings of the Aquaculture international congress, Vancouver, Canada, pp 531–535

  • Wolters WR, Libey GS, Chrisman CL (1982) Effects of triploidy on growth and gonadal development of channel catfish. Trans Am Fish Soc 111:102–105

    Article  Google Scholar 

Download references

Acknowledgments

The authors would like to express their gratitude to University Putra Malaysia (UPM) for providing the facilities to perform the experiments; to Mr. Jasni bin md. Yusoff, the manager of Puchong’s Aquaculture Research Station, for his kind help; Prof. Siti Shapor Siraj and Mrs. Ratiah Sukardi for their kind assistance during chromosomal preparation; and Dr. Tillmann J. Benfey for helpful comments on an earlier draft of the manuscript.

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Authors and Affiliations

  1. Department of Aquaculture, Faculty of Agriculture, University Putra Malaysia, 43400, Selangor, Malaysia

    A. Karami & A. Christianus

  2. Biodiagnosis & Biosafety Programme, Biotechnology Research Centre, Malaysian Agricultural Research and Development Institute, 50774, Kuala Lumpur, Malaysia

    Z. Ishak, M. Noor Azlina & H. Noorshinah

  3. Department of Biology, Fisheries and Oceans Canada at the Canadian Rivers Institute, University of New Brunswick, Fredericton, NB, Canada

    S. C. Courtenay

  4. Department of Biochemistry, Faculty of Biotechnology, University Putra Malaysia, 43400, Selangor, Malaysia

    M. A. Syed

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  1. A. Karami
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  2. A. Christianus
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  4. S. C. Courtenay
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Correspondence to A. Christianus.

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Karami, A., Christianus, A., Ishak, Z. et al. Effect of triploidization on juvenile African catfish (Clarias gariepinus). Aquacult Int 18, 851–858 (2010). https://doi.org/10.1007/s10499-009-9307-x

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  • DOI: https://doi.org/10.1007/s10499-009-9307-x

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