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Vitamin D treatment of senescence accelerated mice (SAM-P/6) induces several regulators of stromal cell plasticity

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Abstract

In an attempt to understand the regulation of bone marrow multipotential cells plasticity in vivo, we treated 4-month-old SAM-P/6 mice with a constant infusion of either 18 pmol/24 h of 1,25(OH)2D3 or vehicle alone for 6 weeks. In vehicle treated animals 78% ± 4 adipose volume vs. total volume was stained positive with oil red O as compared to only 32 ± 3% in 1,25(OH)2D3treated animals (P < 0.001). Furthermore, we aimed to identify the changes in gene expression induced by 1,25(OH)2D3in bone marrow cells by analyzing a set of 5440 genes in the NIA 15K Mouse cDNA microarray. Overall, a coordinated regulation of genes which both stimulate osteoblastogenesis and inhibit adipogenesis was observed in 1,25(OH)2D3-treated mice when compared to vehicle treated mice. In summary, this study illustrates the anti-adipogenic effect of 1,25(OH)2D3in bone cells and identifies some of the possible key signals involved in bone cell plasticity.

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Authors and Affiliations

  1. Division of Geriatric Medicine, McGill University, H3T 1E2, 3755, Cote Sainte Catherine, Montreal, QC, Canada

    Gustavo Duque

  2. Bloomfield Centre for Studies in Aging, Jewish General Hospital, Montreal, Canada

    Gustavo Duque

  3. Centre for Bone and Periodontal Research, McGill University, Montreal, Canada

    Gustavo Duque, Michael Macoritto & Richard Kremer

Authors
  1. Gustavo Duque
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  2. Michael Macoritto
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  3. Richard Kremer
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Correspondence to Gustavo Duque.

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Duque, G., Macoritto, M. & Kremer, R. Vitamin D treatment of senescence accelerated mice (SAM-P/6) induces several regulators of stromal cell plasticity. Biogerontology 5, 421–429 (2004). https://doi.org/10.1007/s10522-004-3192-5

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  • DOI: https://doi.org/10.1007/s10522-004-3192-5

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