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Contrary Effects of BMP-2 and ATRA on Adipogenesis in Mouse Mesenchymal Fibroblasts

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Abstract

This study evaluates the effects of bone morphogenetic protein 2 (BMP-2) and all trans retinoic acid (ATRA) on adipogenesis in primary mouse embryo fibroblasts (MEFs). In BMP-2-treated MEFs, lipid accumulation and substantial induction of the adipocyte specific marker 442-aP2 suggested the conversion of MEFs into adipocytes. Such adipogenesis was found to be mediated through sequential induction of C/EBPα, C/EBPβ, and PPARγ. Both the BMP/Smad and BMP/p38 pathways contributed to the adipocyte differentiation. Contrary to the effects of BMP-2, ATRA was demonstrated to inhibit adipocyte differentiation in MEFs. Semi-quantitative RT-PCR analysis revealed that ATRA caused a selective inhibition of both the basal and induction levels of C/EBPα and PPARγ, without altering the expression pattern of C/EBPβ. Taken together, these data suggest the roles of BMP-2 and ATRA in adipogenic differentiation of primary MEFs, and the possible molecular mechanism that involves the regulation of C/EBPα, C/EBPβ, and PPARγ.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China Nos. 30600328 and 30740030.

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Authors and Affiliations

  1. Institute of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou City, Guangdong, 510632, China

    Fenyong Sun

  2. Medical Research Center, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou City, Guangdong, 510120, China

    Qiuhui Pan & Shanyin Liu

  3. Department of Laboratory Center, Shanghai Tenth People’s Hospital of Tongji University, Shanghai, 200072, China

    Jiayi Wang, Zhi Li & Yongchun Yu

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  1. Fenyong Sun
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  2. Qiuhui Pan
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  3. Jiayi Wang
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  4. Shanyin Liu
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  5. Zhi Li
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  6. Yongchun Yu
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Corresponding author

Correspondence to Yongchun Yu.

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Authors Fenyong Sun, Qiuhui Pan, and Jiayi Wang contributed equally to this work.

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Sun, F., Pan, Q., Wang, J. et al. Contrary Effects of BMP-2 and ATRA on Adipogenesis in Mouse Mesenchymal Fibroblasts. Biochem Genet 47, 789–801 (2009). https://doi.org/10.1007/s10528-009-9277-8

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  • DOI: https://doi.org/10.1007/s10528-009-9277-8

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