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Aberrant Expression of Some Circulating miRNAs in Childhood Acute Lymphoblastic Leukemia

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Abstract

Acute lymphoblastic leukemia (ALL) is a heterogeneous cancer commonly affecting children due to dysregulation of miRNA expression. In the current study, authors investigated the expression profile for miRNA-125b-1 and miRNA-203 among childhood ALL. Blood samples were collected from newly diagnosed childhood ALL and healthy control children. The expression profile for candidate miRNAs was detected using quantitative RT-PCR analysis. Statistical analysis were performed using receiver operating characteristic curve (ROC) to examine the diagnostic efficacy of the two miRNA and their levels among ALL clinicopathological factors and phenotypes. The median expression level for miRNA-125b-1 was significantly high in childhood ALL; while miRNA-203 level was significantly low in childhood ALL as compared to control ones. MiRNA-125-1 reported significant increase in T-ALL as compared to other ALL phenotypes. Median miRNA-203 level was high in T-ALL followed by pre-B-ALL although no significant difference was reported. Clinicopathological factors did not emphasize significance with either detected miRNAs. Using ROC curve the diagnostic efficacy was significant with an area under the curve 0.858 for miRNA-125b-1 (83.72, 100%) and 0.878 for miRNA-203 (97.67, 86.96%). The combination of the two key miRNAs revealed absolute sensitivity (100%). MiRNA-125b-1 and miRNA-203 can be useful molecular markers for diagnosis of ALL. Further studies with large cohort are warranted to validate these results.

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Acknowledgement

This work was partially funded through National Research Centre, Giza, Egypt.

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Authors and Affiliations

  1. Genetic Engineering and Biotechnology Research Division, Biochemistry Department, High Throughput Molecular and Genetic laboratory, Center for Excellences for Advanced Sciences, National Research Centre, El-Bohouth Street, Dokki, Giza, 12622, Egypt

    Menha Swellam, Maha Hashim, Magda Sayed Mahmoud & Amal Ramadan

  2. Department of Biochemistry, Genetic Engineering and Biotechnology Research Division, National Research Centre, Dokki, Giza, Egypt

    Menha Swellam, Maha Hashim, Magda Sayed Mahmoud & Amal Ramadan

  3. Department of Clinical Pathology, National Cancer Institute, Cairo, Egypt

    Naglaa M. Hassan

Authors
  1. Menha Swellam
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  2. Maha Hashim
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  3. Magda Sayed Mahmoud
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  4. Amal Ramadan
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  5. Naglaa M. Hassan
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Correspondence to Menha Swellam.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Swellam, M., Hashim, M., Mahmoud, M.S. et al. Aberrant Expression of Some Circulating miRNAs in Childhood Acute Lymphoblastic Leukemia. Biochem Genet 56, 283–294 (2018). https://doi.org/10.1007/s10528-018-9844-y

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  • DOI: https://doi.org/10.1007/s10528-018-9844-y

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