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Antimicrobial peptide P18 inhibits inflammatory responses by LPS- but not by IFN-γ-stimulated macrophages

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Abstract

Antimicrobial peptide P18 markedly inhibited the expression of inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α) and interleukin-1 beta (IL-1β) in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells, whereas magainin 2 did not inhibit these activities. P18 dose-dependently reduced nitric oxide (NO) production by LPS-stimulated RAW 264.7 macrophage cells, with complete inhibition at 20 μg P18 ml−1. In contrast, P18 had no effect on NO production and the expression of iNOS mRNA and iNOS protein by interferon-gamma (IFN-γ)-stimulated RAW264.7 cells, suggesting P18 selectively inhibits LPS-stimulated inflammatory responses in macrophages. An LAL assay showed that P18 has strong LPS-neutralizing activity, indicating that P18 inhibits the inflammatory responses in LPS-stimulated macrophages by direct binding to LPS. Collectively, our results indicate that P18 has promising therapeutic potential as a novel anti-inflammatory as well as antimicrobial agent.

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Authors and Affiliations

  1. Department of Cellular & Molecular Medicine, School of Medicine, Chosun University, Gwangju, 501-759, Republic of Korea

    Yong Hai Nan, Il-Seon Park & Song Yub Shin

  2. Department of Bio-Materials, Graduate School and Research Center for Proteineous Materials, Chosun University, Gwangju, 501-759, Republic of Korea

    Yong Hai Nan, Il-Seon Park & Song Yub Shin

  3. Department of Pharmacology, School of Medicine, Chosun University, Gwangju, 501-759, Republic of Korea

    Young Jin Jeon

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  1. Yong Hai Nan
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  2. Young Jin Jeon
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  3. Il-Seon Park
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  4. Song Yub Shin
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Correspondence to Song Yub Shin.

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Nan, Y.H., Jeon, Y.J., Park, IS. et al. Antimicrobial peptide P18 inhibits inflammatory responses by LPS- but not by IFN-γ-stimulated macrophages. Biotechnol Lett 30, 1183–1187 (2008). https://doi.org/10.1007/s10529-008-9682-9

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  • DOI: https://doi.org/10.1007/s10529-008-9682-9

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