Abstract
A novel method of one-step preparation of dual-labeled gold nanoparticle bio-probes was established by the electrostatic adsorption and the covalent bonding of gold nanoparticles with antibodies and thiol-modified oligonucleotides, respectively. Characterization of probes, the coverage and activity of antibodies and oligonucleotides on probe surfaces were detected. The results indicated that the gold nanoparticles labeled with antibodies and oligonucleotides possess good bioactivity and the coverage of oligonucleotide and antibody on a dual-labeled gold nanoparticle bio-probe was (92 ± 20) and (8 ± 3), respectively. The preparative method is simple and stable. The dual-labeled gold nanoparticle bio-probes have an application value in detection of ultramicro protein.
Similar content being viewed by others
References
Ackerson CJ, Sykes MT, Kornberg RD (2005) Defined DNA_nanoparticle conjugates. Proc Natl Acad Sci USA 102:13383–13385
Dubertret B, Calame M, Libchaber AJ (2001) Single-mismatch detection using gold-quenched fluorescent oligonucleotides. Nat Biotechnol 19:365–370
Dulkeith E, Ringler M, Klar TA, Feldmann J, Muñoz Javier A, Parak WJ (2005) Gold nanoparticles quench fluorescence by phase induced radiative rate suppression. Nano Lett 5:585–589
Fan C, Wang S, Hong JW, Bazan GC, Plaxco KW, Heeger AJ (2003) Beyond superquenching: hyper-efficient energy transfer from conjugated polymers to gold nanoparticles. Proc Natl Acad Sci USA 100:6297–6301
Frens G (1973) Controlled nucleation for the regulation of the particle size in monodisperse gold solution. Nat Phys Sci 241:20–22
Jennings T, Strouse G (2007) Past, present, and future of gold nanoparticles. Adv Exp Med Biol 620:34–47
Kanaras AG, Wang Z, Bates AD, Cosstick R, Brust M (2003) Towards multistep nanostructure synthesis: programmed enzymatic self-assembly of DNA/gold systems. Angew Chem Int Ed Engl 42:191–194
Li H, Rothberg L (2004) Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles. Proc Natl Acad Sci USA 101:14036–14039
Nam JM, Thaxton CS, Mirkin CA (2003) Nanoparticle-based bio-bar codes for the ultrasensitive detection of proteins. Science 301:1884–1886
Park SJ, Taton TA, Mirkin CA (2002) Array-based electrical detection of DNA with nanoparticle probes. Science 295:1503–1506
Qin WJ, Yung LY (2005) Nanoparticle-DNA conjugates bearing a specific number of short DNA strands by enzymatic manipulation of nanoparticle-bound DNA. Langmuir 21:11330–11334
Stoeva SI, Lee JS, Smith JE, Rosen ST, Mirkin CA (2006a) Multiplexed detection of protein cancer markers with biobarcoded nanoparticle probes. J Am Chem Soc 128:8378–8379
Stoeva SI, Lee JS, Thaxton CS, Mirkin CA (2006b) Multiplexed DNA detection with biobarcoded nanoparticle probes. Angew Chem Int Ed Engl 45:3303–3306
Xiao Y, Patolsky F, Katz E, Hainfeld JF, Willner I (2003) “Plugging into enzymes”: nanowiring of redox enzymes by a gold nanoparticle. Science 299:1877–1881
Acknowledgements
The authors greatly appreciated the financial support from Technology Project of Shenzhen (No. 2004A110) and National 863 High Technology Project (No. 2003BA310A23).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kong, Xl., Qiao, Fy., Qi, H. et al. One-step preparation of antibody and oligonucleotide dual-labeled gold nanoparticle bio-probes and their properties. Biotechnol Lett 30, 2071–2077 (2008). https://doi.org/10.1007/s10529-008-9802-6
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-008-9802-6