Abstract
Tenocytes reside in relatively avascular tissue and are difficult to expand due to phenotype drift and functional loss. Thus low O2 tension culture was employed to enhance the expansion capability. The results demonstrated that low O2 tension (2% O2) culture could significantly enhance the expansion of newborn pig tenocytes with 275–473% greater cell yield per cell passage that that of regular O2 cultured (21% O2) cells. Importantly, low O2 culture did not change the gene expression of functional molecule such as collagens I and III, decorin, prolyl 4-hydroxylase (P4H), lysyl oxidase (LOX), TIMP-1 and TIMP-2, but could significantly down regulate the gene expression of MMP-1 and IL-6. In conclusion, low O2 tension culture can significantly enhance the expansion capacity of tenocytes without affecting their phenotype and functions.
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We appreciate the financial support from “973” grant (2005CB522703) and “863” grant (2006AA02A127) and other grants (09ZR1416700, 2008XJ026).
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Zhang, Y., Wang, B., Zhang, W.J. et al. Enhanced proliferation capacity of porcine tenocytes in low O2 tension culture. Biotechnol Lett 32, 181–187 (2010). https://doi.org/10.1007/s10529-009-0137-8
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DOI: https://doi.org/10.1007/s10529-009-0137-8