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BMP-2, VEGF and bFGF synergistically promote the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells

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Abstract

Mesenchymal stem cells (MSCs) were treated with bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) dose-dependently and time-dependently. Together they caused a strong synergistic effect on the osteogenic differentiation of MSCs, with lower concentrations of each factor being enough to show the synergistic promotion (50 ng BMP-2/ml, 1 ng VEGF/ml and 10 ng bFGF/ml). When both VEGF and bFGF were added in the early proliferating stage (the first 7 days) and BMP-2 was added in the late differentiation stage (the last 7 days), osteogenic differentiation of MSCs could be enhanced more effectively.

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Acknowledgments

This work has been supported by the National Natural Science Foundation of China (51173120) and Key Technologies Research and Development Program of Sichuan Province (2010SZ0088 & 2008SZ0021).

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Authors and Affiliations

  1. College of Materials Science and Engineering, Sichuan University, Chengdu, 610064, People’s Republic of China

    Yan Bai, Peipei Li, Guangfu Yin, Zhongbing Huang, Xiaoming Liao, Xianchun Chen & Yadong Yao

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  1. Yan Bai
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  2. Peipei Li
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  3. Guangfu Yin
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  4. Zhongbing Huang
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  5. Xiaoming Liao
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  6. Xianchun Chen
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  7. Yadong Yao
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Corresponding author

Correspondence to Guangfu Yin.

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Bai, Y., Li, P., Yin, G. et al. BMP-2, VEGF and bFGF synergistically promote the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells. Biotechnol Lett 35, 301–308 (2013). https://doi.org/10.1007/s10529-012-1084-3

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  • DOI: https://doi.org/10.1007/s10529-012-1084-3

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