biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:149-152, 2005 | DOI: 10.1007/s10535-005-0152-0

Indexing and production of virus-free chrysanthemums

R. Ram1, N. Verma1, A. K. Singh1, L. Singh1, V. Hallan1, A. A. Zaidi1,*
1 Molecular Plant Virology Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Palampur, India

Chrysanthemum morifolium cv. Regol Time plants were found to be infected with Chrysanthemum B carlavirus (CVB). They were made CVB-free by using meristem tip culture, chemotherapy and thermotherapy. The plants were indexed by biological assay, double antibody sandwitch enzyme linked immunosorbent assay (DAS-ELISA) and reverse transcriptase polymerase chain reaction (RT-PCR). The maximum number of virus-free plants (26.7 %, as indexed by RT-PCR) was obtained with 2-thiouracil at 0.04 g dm-3 concentration. Only 12 % plants were found to be virus-free, after being kept at 38 °C for 30 d. For indexing CVB in chrysanthemums, RT-PCR was found to be the most reliable method.

Keywords: antiviral compounds; Chrysanthemum B carlavirus; Chrysanthemum morifolium; DAS-ELISA; in vitro production; RT-PCR
Subjects: antiviral compounds; Chrysanthemum B carlavirus (CVB); Chrysanthemum morifolium; reverse transcriptase polymerase chain reaction; virus-free chrysanthemum, indexing

Received: January 26, 2004; Accepted: November 30, 2004; Published: March 1, 2005  Show citation

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Ram, R., Verma, N., Singh, A.K., Singh, L., Hallan, V., & Zaidi, A.A. (2005). Indexing and production of virus-free chrysanthemums. Biologia plantarum, 49(1), 149-152. doi: 10.1007/s10535-005-0152-0
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