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Fabrication of three-dimensional scaffolds for heterogeneous tissue engineering

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Abstract

The development of biomedical scaffolds mimicking a heterogeneous cellular microenvironment for a specified regulation of cell-fates is very promising for tissue engineering. In this study, three-dimensional scaffolds with heterogeneous microstructure were developed using a DMD-PP apparatus. During the fabrication process, this apparatus can efficiently switch monomers to form microstructures with localized, different material properties; the resolution in the arrangement of material properties is comparable to the characteristic size of functional subunits in living organs, namely, a hundred microns. The effectiveness of this DMD-PP apparatus is demonstrated by a woodpile microstructure with heterogeneous fluorescence and also by a microporous cell-culturing scaffold with selected sites for protein adhesion. Cell-cultivation experiment was performed with the microporous scaffold, in which selective cell adhesion was observed.

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Acknowledgement

This work is supported by grants to SC from the Office of Naval Research and the National Science Foundation. We appreciate the donation of the DMD tool kit from Texas Instruments. We also thank the computer support from Intel’s High Education Program.

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Authors and Affiliations

  1. Texas Materials Institute and Department of Mechanical Engineering, The University of Texas at Austin, C2200, Austin, TX, 78712, USA

    Li-Hsin Han & Shaochen Chen

  2. Department of Biomedical Engineering, The University of Texas at Austin, C0800, Austin, TX, 78712, USA

    Shalu Suri & Christine E. Schmidt

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  1. Li-Hsin Han
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  2. Shalu Suri
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  3. Christine E. Schmidt
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  4. Shaochen Chen
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Correspondence to Shaochen Chen.

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Han, LH., Suri, S., Schmidt, C.E. et al. Fabrication of three-dimensional scaffolds for heterogeneous tissue engineering. Biomed Microdevices 12, 721–725 (2010). https://doi.org/10.1007/s10544-010-9425-2

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  • DOI: https://doi.org/10.1007/s10544-010-9425-2

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